Sökning: id:"swepub:oai:gup.ub.gu.se/250460" > On the mechanism of...
Fältnamn | Indikatorer | Metadata |
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000 | 04687naa a2200565 4500 | |
001 | oai:gup.ub.gu.se/250460 | |
003 | SwePub | |
008 | 240910s2016 | |||||||||||000 ||eng| | |
009 | oai:research.chalmers.se:f706da2d-0ab1-4516-8e18-74a7abd96e9d | |
024 | 7 | a https://gup.ub.gu.se/publication/2504602 URI |
024 | 7 | a https://doi.org/10.1039/c6fd00102e2 DOI |
024 | 7 | a https://research.chalmers.se/publication/2476312 URI |
040 | a (SwePub)gud (SwePub)cth | |
041 | a eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Lovric, Jelena,d 1980u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)lovric |
245 | 1 0 | a On the mechanism of electrochemical vesicle cytometry: chromaffin cell vesicles and liposomes |
264 | c 2016 | |
264 | 1 | b Royal Society of Chemistry (RSC),c 2016 |
520 | a The mechanism of mammalian vesicle rupture onto the surface of a polarized carbon fiber microelectrode during electrochemical vesicle cytometry is investigated. It appears that following adsorption to the surface of the polarized electrode, electroporation leads to the formation of a pore at the interface between a vesicle and the electrode and this is shown to be potential dependent. The chemical cargo is then released through this pore to be oxidized at the electrode surface. This makes it possible to quantify the contents as it restricts diffusion away from the electrode and coulometric oxidation takes place. Using a bottom up approach, lipid-only transmitter-loaded liposomes were used to mimic native vesicles and the rupture events occurred much faster in comparison with native vesicles. Liposomes with added peptide in the membrane result in rupture events with a lower duration than that of liposomes and faster in comparison to native vesicles. Diffusional models have been developed and suggest that the trend in pore size is dependent on soft nanoparticle size and diffusion of the content in the nanometer vesicle. In addition, it appears that proteins form a barrier for the membrane to reach the electrode and need to move out of the way to allow close contact and electroporation. The protein dense core in vesicles matrixes is also important in the dynamics of the events in that it significantly slows diffusion through the vesicle. | |
650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
650 | 7 | a NATURVETENSKAPx Kemix Analytisk kemi0 (SwePub)104012 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Chemical Sciencesx Analytical Chemistry0 (SwePub)104012 hsv//eng |
653 | a lipid-bilayer formation | |
653 | a fusion pore | |
653 | a amperometric measurements | |
653 | a catecholamine content | |
653 | a vesicular exocytosis | |
653 | a secretory vesicles | |
653 | a extended kiss | |
653 | a full fusion | |
653 | a release | |
653 | a membrane | |
653 | a Chemistry | |
700 | 1 | a Najafinobar, Neda,d 1985u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)nobar |
700 | 1 | a Dunevall, Johan,d 1984u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)dunevall |
700 | 1 | a Majdi, Soodabeh,d 1980u Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)soodabeh |
700 | 1 | a Svir, I.u Processus d'Activation Selective Par Transfert d'Energie Uni-Electronique ou Radiatif4 aut |
700 | 1 | a Oleinick, A.u Processus d'Activation Selective Par Transfert d'Energie Uni-Electronique ou Radiatif4 aut |
700 | 1 | a Amatore, C.u Processus d'Activation Selective Par Transfert d'Energie Uni-Electronique ou Radiatif4 aut |
700 | 1 | a Ewing, Andrew G,d 1957u Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)andrewe |
710 | 2 | a Chalmers tekniska högskolab Institutionen för kemi och molekylärbiologi4 org |
773 | 0 | t Faraday Discussionsd : Royal Society of Chemistry (RSC)g 193, s. 65-79q 193<65-79x 1359-6640x 1364-5498 |
856 | 4 | u http://dx.doi.org/10.1039/c6fd00102ey FULLTEXT |
856 | 4 8 | u https://gup.ub.gu.se/publication/250460 |
856 | 4 8 | u https://doi.org/10.1039/c6fd00102e |
856 | 4 8 | u https://research.chalmers.se/publication/247631 |
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