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Cloning of two novel growth hormone transcripts expressed in human placenta.
- Boguszewski, C L (författare)
-
- Svensson, Per-Arne, 1969 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för internmedicin,Institute of Internal Medicine, Dept of Medicine
- Jansson, T (författare)
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- Clark, R (författare)
- Carlsson, L M (författare)
- Carlsson, B (författare)
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- (creator_code:org_t)
- The Endocrine Society, 1998
- 1998
- Engelska.
- Ingår i: The Journal of clinical endocrinology and metabolism. - : The Endocrine Society. - 0021-972X .- 1945-7197. ; 83:8, s. 2878-85
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- Several isoforms of human GH (hGH) are produced by two related genes expressed in the pituitary (hGH-N) and in the placenta (hGH-V). These genes consist of five exons (denoted 1-5) separated by four introns (denoted A-D). In the present report, two new transcripts of the hGH-V gene are described. The coding region of the hGH-V gene was amplified by RT-PCR using placental complementary DNA as template. DNA sequencing of several clones revealed two novel transcripts. One had a 45-bp deletion caused by the use of an alternative splice acceptor site within exon 3, similar to that in the hGH-N gene, predicting a 20-kDa isoform of hGH-V. The other transcript was generated by the use of an alternative splice donor site causing a 4-bp deletion in the end of exon 4, predicting a 24-kDa protein with 219 amino acids, which we refer to as hGH-V3. The carboxy-terminal sequence of hGH-V3 differs from 22-kDa hGH-V and hGH-V2, the two previously reported transcripts of the hGH-V gene, and does not contain a predicted transmembrane domain as described for hGH-V2. Ligase chain reaction was then used to analyze the possible use of the same splicing pattern in transcripts derived from the other genes of the hGH-gene cluster. Alternatively spliced transcripts encoding the 20-kDa hGH isoform were detected from the hGH-N and hGH-V genes, but not from the human chorionic somatomammotropin-A/B genes. The alternative splicing generating hGH-V3 was only demonstrated in transcripts derived from the hGH-V gene. Using competitive RT-PCR, the expression of hGH-V3 was estimated to be 10% of the hGH-V messenger RNA in full-term normal placentas and in placentas from pathological pregnancies. The 20-kDa hGH-V was detected in two of four full-term normal placentas, whereas a weak signal was observed in one of the pathological placentas. We conclude that the hGH-V primary transcript undergoes alternative splicing pathways generating at least four different messenger RNAs, predicting the expression of different hGH isoforms, including two with a complete sequence divergence in the carboxy-terminus.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
Nyckelord
- Alternative Splicing
- Amino Acid Sequence
- Base Sequence
- Chemical Phenomena
- Chemistry
- Physical
- Cloning
- Molecular
- DNA
- Complementary
- Female
- Gene Expression
- Genetic Variation
- Human Growth Hormone
- chemistry
- genetics
- Humans
- Molecular Sequence Data
- Placenta
- chemistry
- Polymerase Chain Reaction
- Pregnancy
- RNA
- Messenger
- analysis
- genetics
- RNA-Directed DNA Polymerase
- Sequence Analysis
- DNA
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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- The Journal of clinical endocrinology and metabolism (Sök värdpublikationen i LIBRIS)
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- Boguszewski, C L
- Svensson, Per-Ar ...
- Jansson, T
- Clark, R
- Carlsson, L M
- Carlsson, B
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- Göteborgs universitet
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