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Determination of me...
Determination of mechanical and rheological properties of a cell-loaded peptide gel during ECM production
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- Barreto Henriksson, Helena (author)
- Sahlgrenska universitetssjukhuset,Sahlgrenska University Hospital,Sahlgrenska Hospital, Sweden
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- Llorente, M. (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden
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- Larsson, Anette, 1966 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden
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- Brisby, Helena, 1965 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för ortopedi,Institute of Clinical Sciences, Department of Orthopaedics,University of Gothenburg,University of Gothenburg, Sweden
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- Gold, Julie, 1963 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden
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- Schuster, E. (author)
- RISE,Jordbruk och livsmedel,Chalmers University of Technology, Sweden
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- Ström, Anna, 1976 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden
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(creator_code:org_t)
- Elsevier BV, 2019
- 2019
- English.
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In: International Journal of Pharmaceutics. - : Elsevier BV. - 0378-5173 .- 1873-3476. ; 563, s. 437-444
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Abstract
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- The development of an injectable biomaterial that supports cell survival and maintains or promotes nucleus pulposus (NP) phenotype could aid delivery of cells to degenerated NPs causing low back pain. Mesenchymal cells were loaded and grown in a synthetic peptide gel, PuraMatrix (R). Cells were observed within the gels over 0-28 days, and accumulation of glycosaminoglycans were detected by histological staining. The mechanical properties of the cell-loaded constructs, and the change of the mechanical properties were studied using stress relaxation of the gels under compression and confinement. The PuraMatrix (R) gel was shown to relax fast on compression indicating that the fluid could easily flow out of the gel, and thus indicating the presence of large pores/voids. The presence of these pores/voids was further supported by high mobility of dextran molecules, determined using fluorescence recovery after photo bleaching. The stress required to deform the cell-loaded constructs to a specific strain increases at day 21, at which point the presence of glycosaminoglycans within the cell-loaded constructs was also observed. The results provide evidence of changes in mechanical properties of the PuraMatrix (R) matrix upon excretion of the extracellular matrix by the cells.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
Keyword
- Extracellular matrix
- PuraMatrix
- hMSc
- Stress relaxation
- Glycosaminoglycans
- GAG
- in-vitro chondrogenesis
- mesenchymal stem-cells
- human nucleus pulposus
- low-back-pain
- intervertebral disc
- gene-expression
- hyaluronic-acid
- degeneration
- hydrogels
- scaffold
- PuraMatrix
Publication and Content Type
- ref (subject category)
- art (subject category)
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Barreto Henrikss ...
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Llorente, M.
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Larsson, Anette, ...
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Brisby, Helena, ...
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Gold, Julie, 196 ...
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Schuster, E.
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Ström, Anna, 197 ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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MEDICAL AND HEAL ...
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University of Gothenburg
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Chalmers University of Technology
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RISE