A Genome-Wide Screen in Saccharomyces cerevisiae Reveals a Critical Role for Oxidative Phosphorylation in Cellular Tolerance to Lithium Hexafluorophosphate

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A Genome-Wide Screen in Saccharomyces cerevisiae Reveals a Critical Role for Oxidative Phosphorylation in Cellular Tolerance to Lithium Hexafluorophosphate

Jin, X. J. (author)

Zhang, J. (author)

An, T. T. (author)

Zhao, H. H. (author)

Fu, W. H. (author)

Li, D. Q. (author)

Liu, S. K. (author)

Cao, X. L. (author)

Liu, Beidong, 1972 (author): Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology

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2021-04-13
2021
English.
In: Cells. - : MDPI AG. - 2073-4409. ; 10:4

Related links:: https://doi.org/10.3...; show more...; https://gup.ub.gu.se...; https://doi.org/10.3...; show less...

Journal article (peer-reviewed)

Abstract Subject headings

Lithium hexafluorophosphate (LiPF6) is one of the leading electrolytes in lithium-ion batteries, and its usage has increased tremendously in the past few years. Little is known, however, about its potential environmental and biological impacts. In order to improve our understanding of the cytotoxicity of LiPF6 and the specific cellular response mechanisms to it, we performed a genome-wide screen using a yeast (Saccharomyces cerevisiae) deletion mutant collection and identified 75 gene deletion mutants that showed LiPF6 sensitivity. Among these, genes associated with mitochondria showed the most enrichment. We also found that LiPF6 is more toxic to yeast than lithium chloride (LiCl) or sodium hexafluorophosphate (NaPF6). Physiological analysis showed that a high concentration of LiPF6 caused mitochondrial damage, reactive oxygen species (ROS) accumulation, and ATP content changes. Compared with the results of previous genome-wide screening for LiCl-sensitive mutants, we found that oxidative phosphorylation-related mutants were specifically hypersensitive to LiPF6. In these deletion mutants, LiPF6 treatment resulted in higher ROS production and reduced ATP levels, suggesting that oxidative phosphorylation-related genes were important for counteracting LiPF6-induced toxicity. Taken together, our results identified genes specifically involved in LiPF6-modulated toxicity, and demonstrated that oxidative stress and ATP imbalance maybe the driving factors in governing LiPF6-induced toxicity.

Subject headings

NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)

Keyword

LiPF6
genome-wide screen
mitochondrial damage
ROS
ATP content
oxidative phosphorylation
yeast
mitochondria
mutants
stress
genes
ros
accumulation
dysfunction
sensitivity
annotation
Cell Biology

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ref (subject category)
art (subject category)

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By the author/editor: Jin, X. J.; Zhang, J.; An, T. T.; Zhao, H. H.; Fu, W. H.; Li, D. Q.; show more...; Liu, S. K.; Cao, X. L.; Liu, Beidong, 19 ...; show less...

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NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Cell Biology

Articles in the publication: Cells

By the university: University of Gothenburg

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