Sökning: id:"swepub:oai:gup.ub.gu.se/322294" > The expression of n...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 04571naa a2200541 4500 | |
001 | oai:gup.ub.gu.se/322294 | |
003 | SwePub | |
008 | 240910s2022 | |||||||||||000 ||eng| | |
009 | oai:slubar.slu.se:119810 | |
024 | 7 | a https://gup.ub.gu.se/publication/3222942 URI |
024 | 7 | a https://doi.org/10.1016/j.rvsc.2022.08.0152 DOI |
024 | 7 | a https://res.slu.se/id/publ/1198102 URI |
040 | a (SwePub)gud (SwePub)slu | |
041 | a eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Nystrom, S.4 aut |
245 | 1 0 | a The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay |
264 | 1 | b Elsevier BV,c 2022 |
520 | a Nerve Growth Factor (NGF) is a signalling molecule for pain and inflammation. NGF is increased in synovial fluid from osteoarthritic humans and animals, compared to healthy controls. Monoclonal antibody therapy directed against NGF has been approved to treat pain in osteoarthritic dogs but despite many years of trialling, therapy has not been approved for human use. One reason for this is that adverse reactions with rapidly progressing osteoarthritis has occurred in some individuals. More detailed knowledge of NGF expression in joints is needed. In this study, capillary-based Simple Western was used to analyse NGF in cultured equine chondrocytes. Chondrocytes were collected post mortem from three macroscopically healthy intercarpal joints and three intercarpal joints with mild osteoarthritic changes. The chondrocytes were expanded to passage one and seeded in chondrogenic medium to maintain the phenotype. On day four, cells were either stimulated with LPS or kept untreated in medium. All cells were harvested on day five. Wes analysis of lysates did not show mature NGF but two proforms, 40 and 45 kDa, were identified. Results were confirmed with western blot. The same proforms were expressed in chondrocytes from healthy and osteoarthritic joints. Acute inflammation induced by LPS stimulation did not change the forms of expressed NGF. Capillary Simple Western offers a sensitive and sample -sparing alternative to traditional western blot. However, confirmation of peaks is imperative in order to avoid misinterpretation of findings. In addition, in this case the method did not offer the possibility of quantification advertised by the manufacturers. | |
650 | 7 | a LANTBRUKSVETENSKAPERx Veterinärmedicin0 (SwePub)4032 hsv//swe |
650 | 7 | a AGRICULTURAL SCIENCESx Veterinary Science0 (SwePub)4032 hsv//eng |
650 | 7 | a LANTBRUKSVETENSKAPERx Veterinärmedicinx Patobiologi0 (SwePub)403022 hsv//swe |
650 | 7 | a AGRICULTURAL SCIENCESx Veterinary Sciencex Pathobiology0 (SwePub)403022 hsv//eng |
653 | a Equine | |
653 | a Chondrocyte | |
653 | a Osteoarthritis | |
653 | a Nerve growth factor | |
653 | a Simple western | |
653 | a synovial-fluid | |
653 | a factor ngf | |
653 | a precursor | |
653 | a beta | |
653 | a prongf | |
653 | a trka | |
653 | a Veterinary Sciences | |
700 | 1 | a Kendall, Annau Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health4 aut0 (Swepub:slu)51201 |
700 | 1 | a Adepu, S.u Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health4 aut |
700 | 1 | a Lindahl, Anders,d 1954u Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för laboratoriemedicin,Department of Laboratory Medicine4 aut0 (Swepub:gu)xlandy |
700 | 1 | a Skiöldebrand, Evau Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health4 aut0 (Swepub:slu)50022 |
710 | 2 | a Sveriges lantbruksuniversitetb Inst för biomedicin och veterinär folkhälsovetenskap4 org |
710 | 2 | a Sveriges lantbruksuniversitet |
773 | 0 | t Research in Veterinary Scienced : Elsevier BVg 151, s. 156-163q 151<156-163x 0034-5288x 1532-2661 |
856 | 4 | u https://pub.epsilon.slu.se/id/eprint/29819/contentsx primaryx Raw objectx freey FULLTEXT |
856 | 4 8 | u https://gup.ub.gu.se/publication/322294 |
856 | 4 8 | u https://doi.org/10.1016/j.rvsc.2022.08.015 |
856 | 4 8 | u https://res.slu.se/id/publ/119810 |
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
Kopiera och spara länken för att återkomma till aktuell vy