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Production of exopo...
Production of exopolysaccharide by Burkholderia cenocepacia results in altered cell-surface interactions and altered bacterial clearance in mice
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Conway, B. A. (författare)
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Chu, K. K. (författare)
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- Bylund, Johan, 1975 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för reumatologi och inflammationsforskning,Institute of Internal Medicine, Dept of Rheumatology and Inflammation Research
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visa fler...
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Altman, E. (författare)
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Speert, D. P. (författare)
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visa färre...
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(creator_code:org_t)
- 2004
- 2004
- Engelska.
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Ingår i: J Infect Dis. ; 190:5, s. 957-66
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- Despite the characterization of some Burkholderia cepacia complex exopolysaccharides (EPSs), little is known about the role of EPSs in the pathogenicity of B. cepacia complex organisms. We describe 2 Burkholderia cenocepacia (genomovar III) isolates obtained from a patient with cystic fibrosis (CF): the nonmucoid isolate C8963 and the mucoid isolate C9343. Both isolates had identical random amplified polymorphic DNA patterns. C9343 produced a capsule composed of the EPSs PS-I and PS-II, as well as alpha -1,6-glucan. These isolates exhibited several phenotypic differences: C8963 synthesized octanoyl-homoserine lactone and produced biofilms, but C9343 did not; in a mouse model of pulmonary infection, C8963 was cleared more rapidly than was C9343; and C9343 interacted poorly with macrophages and neutrophils, compared with C8963, suggesting that the C9343 capsule interfered with cell-surface interactions. Overproduction of EPS by C9343 resulted in a mucoid appearance and interfered with cell-surface interactions and clearance in an animal model. This mucoid colonial appearance could enhance the persistence and virulence of this important CF-related pathogen.
Nyckelord
- 4-Butyrolactone/analogs & derivatives/biosynthesis
- Animals
- Bacterial Capsules/*metabolism
- Biofilms/growth & development
- Burkholderia Infections/immunology/microbiology
- development/isolation & purification/*pathogenicity
- Cystic Fibrosis/*immunology/*microbiology
- Disease Models
- Animal
- Electrophoresis
- Gel
- Pulsed-Field
- Female
- Humans
- Mice
- Mice
- Inbred BALB C
- Random Amplified Polymorphic DNA Technique
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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