Nonradioactive in situ hybridization on frozen sections and whole mounts.

Asp, Julia, 1973 (author): Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för patologi,Institute of Biomedicine, Department of Pathology

Abramsson, Alexandra, 1973 (author): Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för patologi,Institute of Biomedicine, Department of Pathology

Betsholtz, Christer, 1959 (author)

(creator_code:org_t)

2006
2006
English.
In: Methods in molecular biology (Clifton, N.J.). - 1064-3745. ; 326, s. 89-102

Abstract Subject headings

Nonradioactive in situ hybridization offers a unique opportunity to study gene expression on samples with preserved histological information. This method makes it possible to locate not only where in a tissue a particular gene is expressed, but in many cases also in which specific cell type it is active. Here, we describe our current protocols for in situ hybridization on frozen sections or whole mounts of mouse embryos. The protocols included describe synthesis of a digoxigenin-labeled probe, tissue handling, hybridization of the probe to the mRNA expressed in the sample and signal detection.

By the author/editor: Asp, Julia, 1973; Abramsson, Alexa ...; Betsholtz, Chris ...

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