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DNA adopts normal B...
DNA adopts normal B-form upon incorporation of highly fluorescent DNA base analogue tC: NMR structure and UV-Vis spectroscopy characterization
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- Engman, Cecilia, 1974 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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- Sandin, Peter, 1977 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Osborne, Sadie (författare)
- University of Southampton
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- Brown, Tom (författare)
- University of Southampton
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- Billeter, Martin, 1955 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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- Lincoln, Per, 1958 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Nordén, Bengt, 1945 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Albinsson, Bo, 1963 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Wilhelmsson, Marcus, 1974 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- Oxford University Press (OUP), 2004
- 2004
- Engelska.
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Ingår i: Nucleic Acids Research. - : Oxford University Press (OUP). - 1362-4962 .- 0305-1048. ; 32:17, s. 5087-95
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Abstract
Ämnesord
Stäng
- The influence of the highly fluorescent tricyclic cytosine base analogue (tC) on duplex DNA conformation is investigated. The duplex properties are characterized by absorbance and circular dichroism (CD) for all combinations of neighbouring bases to tC, and an NMR structure is determined for one tC-containing sequence. For the oligonucleotides with one tC incorporated instead of cytosine, the melting temperature is increased on average by 2.7 degrees C above that for the unmodified ones. CD spectra are practically identical for modified and unmodified sequences, indicating an unperturbed B-DNA conformation. The NMR structure determination of the self-complementary sequence 5'-CTC(tC)ACGTGGAG shows a DNA conformation consistent with B-form for the whole duplex. The root-mean-square distance for the nucleotides of the eight central base pairs between the 10 structures with lowest CYANA target functions and a mean structure is 0.45 +/- 0.17 A. The NMR data confirm correct base pairing for tC by the observation of both intrastrand and interstrand imino proton NOEs. Altogether, this suggests that tC works well as a cytosine analogue, i.e. it is situated in the base stack, forming hydrogen bonds with G in the complementary strand, without distorting the DNA backbone conformation. This first example of an artificial, highly fluorescent DNA base that does not perturb the DNA conformation could have valuable applications for the study of the structure and dynamics of nucleic acid systems.
Ämnesord
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Circular Dichroism
- DNA
- chemistry
- Fluorescent Dyes
- chemistry
- Nuclear Magnetic Resonance
- Biomolecular
- Nucleic Acid Conformation
- Phenothiazines
- chemistry
- Spectrophotometry
- Spectrophotometry
- Ultraviolet
- Temperature
- Spectrophotometry
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Engman, Cecilia, ...
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Sandin, Peter, 1 ...
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Osborne, Sadie
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Brown, Tom
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Billeter, Martin ...
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Lincoln, Per, 19 ...
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visa fler...
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Nordén, Bengt, 1 ...
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Albinsson, Bo, 1 ...
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Wilhelmsson, Mar ...
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- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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och Fysikalisk kemi
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
- Artiklar i publikationen
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Nucleic Acids Re ...
- Av lärosätet
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Göteborgs universitet
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Chalmers tekniska högskola