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Transfer of immune ...
Transfer of immune components from rabbit autoimmune cardiomyopathy into severe combined immunodeficiency (SCID) mice induces cardiomyopathic changes.
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Matsui, Shinobu (author)
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- Fu, Michael, 1963 (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Wallenberg Laboratory,Institute of Medicine, Department of Molecular and Clinical Medicine
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Hayase, Mituru (author)
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Katsuda, Shogo (author)
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Yamaguchi, Nobuo (author)
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Teraoka, Kohei (author)
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Kurihara, Takayuki (author)
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Murano, Hidekazu (author)
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Takekoshi, Noboru (author)
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(creator_code:org_t)
- 2009-07-07
- 2006
- English.
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In: Autoimmunity. - : Informa UK Limited. - 0891-6934 .- 1607-842X. ; 39:2, s. 121-8
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Abstract
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- BACKGROUND: Growing evidence suggests that autoimmune mechanism plays an important role in the pathogenesis of cardiomyopathy. The purpose of this study was to investigate whether passive transfer of IgG and/or lymphocytes from rabbits with autoimmune cardiomyopathy is able to reproduce cardiomyopathic changes in severe combined immunodeficiency (SCID) mice. METHODS AND RESULTS: SCID mice were injected intraperitoneally with IgG and/or peripheral blood lymphocytes (PBL) from either rabbits immunized with both beta1-adrenoceptor peptide and M2-muscarinic receptor peptide (beta1+M2 group) or rabbits with adjuvant (N group). Thirty five SCID mice were divided into seven groups; N-IgG, N-PBL, N-IgG & PBL, (beta1+M2)-IgG, (beta1+M2)-PBL, (beta1+M2)-IgG & PBL and control groups. Heart weight in three (beta1+M2) groups were significantly increased. All mice in three (beta1+M2) groups showed high titer of rabbit anti-beta1 adrenoceptor autoantibodies, and 4 mice in the (beta1+M2)-PBL group and 3 mice in the (beta1+M2)-IgG & PBL group showed a significant increase in titer of rabbit anti-M2-muscarinic receptor autoantibodies. Focal infiltration of inflammatory cells in the myocardium was observed in the (beta1+M2)-IgG & PBL group. In the (beta1+M2)-PBL group and (beta1+M2)-IgG & PBL group, cardiomyocyte diameters were significantly increased. Some myocytes of the (beta1+M2)-IgG & PBL group exhibited intracellular edema, clumps of Z-band and increased numbers of mitochondria by using electron microscopy. CONCLUSION: Transfer of IgG and PBL from rabbits immunized with combined beta1 and M2 peptides was able to reproduce the early stage of cardiomyopathic changes in SCID mice.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Amino Acid Sequence
- Animals
- Autoimmune Diseases
- etiology
- immunology
- pathology
- Cardiomyopathies
- etiology
- immunology
- pathology
- Female
- Humans
- Immunization
- Immunization
- Passive
- Male
- Mice
- Mice
- SCID
- Microscopy
- Electron
- Molecular Sequence Data
- Peptide Fragments
- genetics
- immunology
- Rabbits
- Receptor
- Muscarinic M2
- genetics
- immunology
- Receptors
- Adrenergic
- beta-1
- genetics
- immunology
- Recombinant Proteins
- genetics
- immunology
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Matsui, Shinobu
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Fu, Michael, 196 ...
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Hayase, Mituru
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Katsuda, Shogo
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Yamaguchi, Nobuo
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Teraoka, Kohei
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show more...
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Kurihara, Takayu ...
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Murano, Hidekazu
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Takekoshi, Nobor ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Microbiology in ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Autoimmunity
- By the university
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University of Gothenburg