id:"swepub:oai:gup.ub.gu.se/78287"
Search: id:"swepub:oai:gup.ub.gu.se/78287" > Characterization of...
- 1 of 1
- Previous record
- Next record
- To hitlist
Characterization of putative ligands for a fish gonadal androgen receptor in a pulp mill effluent.
-
- Larsson, D. G. Joakim, 1969 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi,Institute of Neuroscience and Physiology, Department of Physiology
- Adolfsson-Erici, Margaretha (author)
- Thomas, Peter (author)
- (creator_code:org_t)
- 2006
- 2006
- English.
- In: Environmental toxicology and chemistry / SETAC. - 0730-7268. ; 25:2, s. 419-27
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Fish exposed to pulp and paper mill effluents often become masculinized. A plausible hypothesis is that this is caused by activation of androgen receptors. The present study aimed to investigate if ligands for the fish gonadal androgen receptor (AR2) are present in pulp mill effluent and to characterize/identify these compounds. Extracts of both primary and biologically treated effluents from a Swedish kraft pulp mill were fractionated chemically. Fractions were tested in competitive binding assays for AR2 from ovaries of the Atlantic croaker (Micropogonias undulatus). Primary effluent contained 96 ng dihydrotestosterone equivalents/ L, whereas biologically treated effluent was 16 times less potent. Further fractionations and assays of binding activities were performed on the primary effluent. Eight final fractions displaced androgen in the binding assay, and gas chromatography/mass spectrometry (GC/MS) analyses revealed that these contained 37 detectable compounds that were not present in inactive fractions. The majority were moderately polar compounds between 200 and 400 g/mol with hydroxyl/carbonyl groups. Two compounds were ruled out because of their lack of binding to AR2. The mass spectra of a third compound matched that of 4-hydroxy-3 (2-(4-hydroxy-3-methoxophenyl)ethyl)-5-metoxyacetophenon, but the remaining candidates could not be fully identified. A search for 21 known steroidal AR2 ligands showed that progesterone, a relatively strong AR2 ligand, was present in the primary effluent (1.6 microg/L) but was removed during the biological treatment step. The detection of multiple fractions with significant binding activity indicates that a variety of compounds in effluents have the potential to masculinize fish near pulp mills via an androgen receptor-mediated mechanism.
Subject headings
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- Animals
- Binding
- Competitive
- Female
- Gas Chromatography-Mass Spectrometry
- Industrial Waste
- Ligands
- Ovary
- metabolism
- Paper
- Perciformes
- physiology
- Receptors
- Androgen
- metabolism
- Sex Differentiation Disorders
- chemically induced
- veterinary
- Water Pollutants
- Chemical
- analysis
- toxicity
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Environmental toxicology and chemistry / SETAC (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Larsson, D. G. J ...
- Adolfsson-Erici, ...
- Thomas, Peter
- About the subject
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Biological Scien ...
- and Microbiology
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Chemical Science ...
- Articles in the publication
- Environmental to ...
- By the university
- University of Gothenburg
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
