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Rapid Detection of ...
Rapid Detection of Multiple Classes of β-Lactam Antibiotics in Blood Using an NDM-1 Biosensing Assay
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- Meng, Qinglai (author)
- Shanxi University
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- Wang, Yao (author)
- Shanxi University
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- Long, Yali (author)
- Shanxi University
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- Yue, Aiping (author)
- Omik Bioscience AB
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- Mecklenburg, Michael (author)
- Omik Bioscience AB
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- Tian, Shuaiyan (author)
- Shanxi University
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- Fu, Yujia (author)
- Shanxi University
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- Yao, Xiangyu (author)
- Shanxi University
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- Liu, Jianyi (author)
- National Institute of Metrology China
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- Song, Dewei (author)
- National Institute of Metrology China
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- Wu, Changxin (author)
- Shanxi University
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- Xie, Bin (author)
- Lund University,Lunds universitet,Tillämpad biokemi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Pure and Applied Biochemistry,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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(creator_code:org_t)
- 2021-09-14
- 2021
- English.
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In: Antibiotics. - : MDPI AG. - 2079-6382. ; 10:9
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Abstract
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- Currently, assays for rapid therapeutic drug monitoring (TDM) of β-lactam antibiotics in blood, which might be of benefit in optimizing doses for treatment of critically ill patients, remain challenging. Previously, we developed an assay for determining the penicillin-class antibiotics in blood using a thermometric penicillinase biosensor. The assay eliminates sample pretreatment, which makes it possible to perform semicontinuous penicillin determinations in blood. However, penicillinase has a narrow substrate specificity, which makes it unsuitable for detecting other classes of β-lactam antibiotics, such as cephalosporins and carbapenems. In order to assay these classes of clinically useful antibiotics, a novel biosensor was developed using New Delhi metallo-β-lactamase-1 (NDM-1) as the biological recognition layer. NDM-1 has a broad specificity range and is capable of hydrolyzing all classes of β-lactam antibiotics in high efficacy with the exception of monobactams. In this study, we demonstrated that the NDM-1 biosensor was able to quantify multiple classes of β-lactam antibiotics in blood plasma at concentrations ranging from 6.25 mg/L or 12.5 mg/L to 200 mg/L, which covered the therapeutic concentration windows of the tested antibiotics used to treat critically ill patients. The detection of ceftazidime and meropenem was not affected by the presence of the β-lactamase inhibitors avibactam and vaborbactam, respectively. Furthermore, both free and protein-bound β-lactams present in the antibiotic-spiked plasma samples were detected by the NDM-1 biosensor. These results indicated that the NDM-1 biosensor is a promising technique for rapid TDM of total β-lactam antibiotics present in the blood of critically ill patients.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Meng, Qinglai
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Wang, Yao
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Long, Yali
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Yue, Aiping
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Mecklenburg, Mic ...
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Tian, Shuaiyan
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show more...
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Fu, Yujia
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Yao, Xiangyu
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Liu, Jianyi
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Song, Dewei
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Wu, Changxin
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Xie, Bin
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
- Articles in the publication
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Antibiotics
- By the university
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Lund University