SwePub
Sök i LIBRIS databas

  Extended search

id:"swepub:oai:lup.lub.lu.se:379eef6d-074f-4089-b46d-c90c20be5ef8"
 

Search: id:"swepub:oai:lup.lub.lu.se:379eef6d-074f-4089-b46d-c90c20be5ef8" > Complex-centric pro...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Complex-centric proteome profiling by SEC-SWATH-MS for the parallel detection of hundreds of protein complexes

Bludau, Isabell (author)
Max Planck Institute of Biochemistry,ETH Zürich
Heusel, Moritz (author)
Lund University,Lunds universitet,Infection Medicine Proteomics,Forskargrupper vid Lunds universitet,Lund University Research Groups,ETH Zürich
Frank, Max (author)
ETH Zürich,European Molecular Biology Laboratory Heidelberg
show more...
Rosenberger, George (author)
ETH Zürich,Columbia University
Hafen, Robin (author)
ETH Zürich
Banaei-Esfahani, Amir (author)
ETH Zürich
van Drogen, Audrey (author)
ETH Zürich
Collins, Ben C. (author)
Queen's University Belfast,ETH Zürich
Gstaiger, Matthias (author)
ETH Zürich
Aebersold, Ruedi (author)
ETH Zürich,University of Zurich
show less...
 (creator_code:org_t)
2020-07-20
2020
English 46 s.
In: Nature Protocols. - : Springer Science and Business Media LLC. - 1754-2189 .- 1750-2799. ; 15:8, s. 2341-2386
  • Journal article (peer-reviewed)
Abstract Subject headings
Close  
  • Most catalytic, structural and regulatory functions of the cell are carried out by functional modules, typically complexes containing or consisting of proteins. The composition and abundance of these complexes and the quantitative distribution of specific proteins across different modules are therefore of major significance in basic and translational biology. However, detection and quantification of protein complexes on a proteome-wide scale is technically challenging. We have recently extended the targeted proteomics rationale to the level of native protein complex analysis (complex-centric proteome profiling). The complex-centric workflow described herein consists of size exclusion chromatography (SEC) to fractionate native protein complexes, data-independent acquisition mass spectrometry to precisely quantify the proteins in each SEC fraction based on a set of proteotypic peptides and targeted, complex-centric analysis where prior information from generic protein interaction maps is used to detect and quantify protein complexes with high selectivity and statistical error control via the computational framework CCprofiler (https://github.com/CCprofiler/CCprofiler). Complex-centric proteome profiling captures most proteins in complex-assembled state and reveals their organization into hundreds of complexes and complex variants observable in a given cellular state. The protocol is applicable to cultured cells and can potentially also be adapted to primary tissue and does not require any genetic engineering of the respective sample sources. At present, it requires ~8 d of wet-laboratory work, 15 d of mass spectrometry measurement time and 7 d of computational analysis.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)

Publication and Content Type

art (subject category)
ref (subject category)

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view