Search: id:"swepub:oai:lup.lub.lu.se:46a8f2fa-5fd8-46ce-ba43-b09ab6c1acdc" > Stress induced biof...
Fältnamn | Indikatorer | Metadata |
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000 | 03719naa a2200373 4500 | |
001 | oai:lup.lub.lu.se:46a8f2fa-5fd8-46ce-ba43-b09ab6c1acdc | |
003 | SwePub | |
008 | 190627s2019 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/46a8f2fa-5fd8-46ce-ba43-b09ab6c1acdc2 URI |
024 | 7 | a https://doi.org/10.1007/s11274-019-2679-92 DOI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Cavero Olguin, Victor Hugou Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,Higher University of San Andrés4 aut0 (Swepub:lu)biot-hcr |
245 | 1 0 | a Stress induced biofilm formation in Propionibacterium acidipropionici and use in propionic acid production. |
264 | c 2019-06-24 | |
264 | 1 | b Springer Science and Business Media LLC,c 2019 |
300 | a 12 s. | |
520 | a Propionibacterium acidipropionici produces propionic acid from different sugars and glycerol; the production can be improved by high cell density fermentations using immobilized cells that help to overcome the limitations of the non-productive lag phase and product inhibition. In this study, the use of stress factors to induce P. acidipropionici to form biofilm and its use as an immobilization procedure in fermentations in bioreactors for producing propionic acid was investigated. Citric acid and sodium chloride increased exopolysaccharide production, biofilm capacity index and trehalose production. Analysis of the expression of trehalose synthesis-related genes otsA and treY by RT-qPCR showed significantly increased expression of only treY during log phase with citric acid, while FISH analysis showed expression of treY and luxS under the influence of both stress factors. The stress factors were then used for development of microbial biofilms as immobilization procedure on Poraver® and AnoxKaldnes® carriers in recycle batch reactors for propionic acid production from 20 g/L glycerol. Highest productivities of 0.7 and 0.78 g/L/h were obtained in Poraver® reactors, and 0.39 and 0.43 g/L/h in AnoxKaldnes® reactors with citric acid and NaCl, respectively. | |
650 | 7 | a TEKNIK OCH TEKNOLOGIERx Industriell bioteknik0 (SwePub)2092 hsv//swe |
650 | 7 | a ENGINEERING AND TECHNOLOGYx Industrial Biotechnology0 (SwePub)2092 hsv//eng |
653 | a Biofilm; immobilization; propionic acid; stress factor; trehalose | |
700 | 1 | a Hatti-Kaul, Rajniu Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biot-rhk |
700 | 1 | a Cardenas, Oscar Victoru Higher University of San Andrés4 aut |
700 | 1 | a Valverde, Marcelo Gutierrezu Higher University of San Andrés4 aut |
700 | 1 | a Romero, Danitza Xiomarau Higher University of San Andrés4 aut |
700 | 1 | a Alvarez-Aliaga, Maria Teresau Higher University of San Andrés4 aut |
710 | 2 | a Bioteknikb Centrum för tillämpade biovetenskaper4 org |
773 | 0 | t World Journal of Microbiology and Biotechnologyd : Springer Science and Business Media LLCg 35:101q 35:101x 0959-3993x 1573-0972 |
856 | 4 | u http://dx.doi.org/10.1007/s11274-019-2679-9y FULLTEXT |
856 | 4 8 | u https://lup.lub.lu.se/record/46a8f2fa-5fd8-46ce-ba43-b09ab6c1acdc |
856 | 4 8 | u https://doi.org/10.1007/s11274-019-2679-9 |
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