Sökning: id:"swepub:oai:lup.lub.lu.se:5a18cd91-20ac-44b1-bcbd-a8e13755ab58" > Uncoupling key dete...
Fältnamn | Indikatorer | Metadata |
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000 | 03732naa a2200397 4500 | |
001 | oai:lup.lub.lu.se:5a18cd91-20ac-44b1-bcbd-a8e13755ab58 | |
003 | SwePub | |
008 | 210818s2021 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/5a18cd91-20ac-44b1-bcbd-a8e13755ab582 URI |
024 | 7 | a https://doi.org/10.1016/j.stemcr.2021.05.0192 DOI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Miharada, Natsumiu Lund University,Lunds universitet,Avdelningen för molekylärmedicin och genterapi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Molecular Medicine and Gene Therapy,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)med-nmh |
245 | 1 0 | a Uncoupling key determinants of hematopoietic stem cell engraftment through cell-specific and temporally controlled recipient conditioning |
264 | 1 | b Elsevier BV,c 2021 |
300 | a 13 s. | |
520 | a Hematopoietic stem cells (HSCs) are typically characterized by transplantation into irradiated hosts in a highly perturbed microenvironment. Here, we show that selective and temporally controlled depletion of resident HSCs through genetic deletion of Gata2 constitutes efficient recipient conditioning for transplantation without irradiation. Strikingly, we achieved robust engraftment of donor HSCs even when delaying Gata2 deletion until 4 weeks after transplantation, allowing homing and early localization to occur in a completely non-perturbed environment. When HSCs from the congenic strains Ly5.1 and Ly5.2 were competitively transplanted, we found that the more proliferative state of Ly5.2 HSCs was associated with superior long-term engraftment when using conditioning by standard irradiation, while higher CXCR4 expression and a better homing ability of Ly5.1 HSCs strongly favored the outcome in our inducible HSC depletion model. Thus, the mode and timing of recipient conditioning challenges distinct functional features of transplanted HSCs. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Hematologi0 (SwePub)302022 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Hematology0 (SwePub)302022 hsv//eng |
653 | a Cxcr4 | |
653 | a engraftment | |
653 | a Gata2 | |
653 | a hematopoietic stem cells | |
653 | a transplantation | |
700 | 1 | a Rydström, Annau Lund University,Lunds universitet,Avdelningen för molekylärmedicin och genterapi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Molecular Medicine and Gene Therapy,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)med-aro |
700 | 1 | a Rak, Justynau University of Cambridge4 aut0 (Swepub:lu)med-jyr |
700 | 1 | a Larsson, Jonasu Lund University,Lunds universitet,Avdelningen för molekylärmedicin och genterapi,Institutionen för laboratoriemedicin,Medicinska fakulteten,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Division of Molecular Medicine and Gene Therapy,Department of Laboratory Medicine,Faculty of Medicine,LUCC: Lund University Cancer Centre,Other Strong Research Environments4 aut0 (Swepub:lu)molm-jla |
710 | 2 | a Avdelningen för molekylärmedicin och genterapib Institutionen för laboratoriemedicin4 org |
773 | 0 | t Stem Cell Reportsd : Elsevier BVg 16:7, s. 1705-1717q 16:7<1705-1717x 2213-6711 |
856 | 4 | u http://dx.doi.org/10.1016/j.stemcr.2021.05.019x freey FULLTEXT |
856 | 4 | u http://www.cell.com/article/S2213671121002708/pdf |
856 | 4 8 | u https://lup.lub.lu.se/record/5a18cd91-20ac-44b1-bcbd-a8e13755ab58 |
856 | 4 8 | u https://doi.org/10.1016/j.stemcr.2021.05.019 |
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