Crystallization and preliminary X-ray analysis of tubulin-folding cofactor A from Arabidopsis thaliana

• Tubulin-folding cofactor A (TFC A) is a molecular post-chaperonin that is involved in the beta-tubulin-folding pathway. It has been identified in many organisms including yeasts, humans and plants. In this work, Arabidopsis thaliana TFC A was expressed in Escherichia coli and purified to homogeneity. After thrombin cleavage, a well diffracting crystal was obtained by the sitting-drop vapour-diffusion method at 289 K. The crystal diffracted to 1.6 A resolution using synchrotron radiation and belonged to space group I4(1), with unit-cell parameters a=55.0, b=55.0, c=67.4 A.

Keyword

Arabidopsis

Arabidopsis Proteins

Crystallization

Crystallography, X-Ray

Molecular Chaperones

Publication and Content Type

art (subject category)

ref (subject category)