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Bacterial glycosida...
Bacterial glycosidases for the production of universal red blood cells
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Liu, Qiyong P. (author)
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Sulzenbacher, Gerlind (author)
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Yuan, Huaiping (author)
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Bennett, Eric P. (author)
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Pietz, Greg (author)
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Saunders, Kristen (author)
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Spence, Jean (author)
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Nudelman, Edward (author)
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Levery, Steven B. (author)
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White, Thayer (author)
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Neveu, John M. (author)
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Lane, William S. (author)
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Bourne, Yves (author)
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- Olsson, Martin L (author)
- Lund University,Lunds universitet,Avdelningen för hematologi och transfusionsmedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Transfusionsmedicin,Forskargrupper vid Lunds universitet,Division of Hematology and Transfusion Medicine,Department of Laboratory Medicine,Faculty of Medicine,Transfusion Medicine,Lund University Research Groups
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Henrissat, Bernard (author)
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Clausen, Henrik (author)
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(creator_code:org_t)
- 2007-04-01
- 2007
- English.
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In: Nature Biotechnology. - : Springer Science and Business Media LLC. - 1546-1696 .- 1087-0156. ; 25:4, s. 454-464
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http://dx.doi.org/10...
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Abstract
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- Enzymatic removal of blood group ABO antigens to develop universal red blood cells ( RBCs) was a pioneering vision originally proposed more than 25 years ago. Although the feasibility of this approach was demonstrated in clinical trials for group B RBCs, a major obstacle in translating this technology to clinical practice has been the lack of efficient glycosidase enzymes. Here we report two bacterial glycosidase gene families that provide enzymes capable of efficient removal of A and B antigens at neutral pH with low consumption of recombinant enzymes. The crystal structure of a member of the alpha-N-acetylgalactosaminidase family reveals an unusual catalytic mechanism involving NAD(+). The enzymatic conversion processes we describe hold promise for achieving the goal of producing universal RBCs, which would improve the blood supply while enhancing the safety of clinical transfusions.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Hematologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Hematology (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Liu, Qiyong P.
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Sulzenbacher, Ge ...
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Yuan, Huaiping
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Bennett, Eric P.
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Pietz, Greg
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Saunders, Kriste ...
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show more...
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Spence, Jean
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Nudelman, Edward
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Levery, Steven B ...
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White, Thayer
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Neveu, John M.
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Lane, William S.
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Bourne, Yves
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Olsson, Martin L
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Henrissat, Berna ...
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Clausen, Henrik
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Hematology
- Articles in the publication
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Nature Biotechno ...
- By the university
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Lund University