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Sökning: id:"swepub:oai:lup.lub.lu.se:869f46a7-e753-46b8-9762-cddb79cee11e" > M1 Macrophages Are ...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00005637naa a2200517 4500
001oai:lup.lub.lu.se:869f46a7-e753-46b8-9762-cddb79cee11e
003SwePub
008170220s2017 | |||||||||||000 ||eng|
0247 a https://lup.lub.lu.se/record/869f46a7-e753-46b8-9762-cddb79cee11e2 URI
0247 a https://doi.org/10.1016/j.jsxm.2016.12.0122 DOI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Matsui, Hotakau Johns Hopkins University,Doai Memorial Hospital,University of Tokyo4 aut
2451 0a M1 Macrophages Are Predominantly Recruited to the Major Pelvic Ganglion of the Rat Following Cavernous Nerve Injury
264 c 2017-02-01
264 1b Oxford University Press (OUP),c 2017
300 a 9 s.
520 a Introduction Neurogenic erectile dysfunction is a common sequela of radical prostatectomy. The etiology involves injury to the autonomic cavernous nerves, which arise from the major pelvic ganglion (MPG), and subsequent neuroinflammation, which leads to recruitment of macrophages to the injury site. Currently, two macrophage phenotypes are known: neurotoxic M1 macrophages and neuroprotective M2 macrophages. Aim To examine whether bilateral cavernous nerve injury (BCNI) in a rat model of erectile dysfunction would increase recruitment of neurotoxic M1 macrophages to the MPG. Methods Male Sprague-Dawley rats underwent BCNI and the MPG was harvested at various time points after injury. The corpora cavernosa was used to evaluate tissue myographic responses to electrical field stimulation ex vivo. Quantitative real-time polymerase chain reaction was used to examine the gene expression of global macrophage markers, M1 macrophage markers, M2 macrophage markers, and cytokines and chemokines in the MPG. Mathematical calculation of the M1/M2 index was used to quantify macrophage changes temporally. Western blot of MPG tissues was used to evaluate the protein amount of M1 and M2 macrophage markers quantitatively. Immunohistochemistry staining of MPGs for CD68, CD86, and CD206 was used to characterize M1 and M2 macrophage infiltration. Main Outcome Measures Corpora cavernosa responsiveness ex vivo; gene (quantitative real-time polymerase chain reaction) and protein (western blot) expressions of M1 and M2 markers, cytokines, and chemokines; and immunohistochemical localization of M1 and M2 macrophages. Results BCNI impaired the corporal parasympathetic-mediated relaxation response to electrical field stimulation and enhanced the contraction response to electrical field stimulation. Gene expression of proinflammatory (Il1b, Il16, Tnfa, Tgfb, Ccl2, Ccr2) and anti-inflammatory (Il10) cytokines was upregulated in the MPG 48 hours after injury. M1 markers (CD86, inducible nitric oxide synthase, interleukin-1β) and M2 markers (CD206, arginase-1, interleukin-10) were increased after BCNI in the MPG, with the M1/M2 index above 1.0 indicating that more M1 than M2 macrophages were recruited to the MPG. Protein expression of the M1 macrophage marker (inducible nitric oxide synthase) was increased in MPGs after BCNI. However, the protein amount of M2 macrophage markers (arginase-1) remained unchanged. Immunohistochemical characterization demonstrated predominant increases in M1 (CD68+CD86+) macrophages in the MPG after BCNI. Conclusion These results suggest that an increase in M1 macrophage infiltration of the MPG after BCNI is associated with impaired neurogenically mediated erectile tissue physiology ex vivo and thus has significant implications for cavernous nerve axonal repair. Future studies are needed to demonstrate that inhibition of M1 macrophage recruitment prevents erectile dysfunction after CNI.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Urologi och njurmedicin0 (SwePub)302142 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Urology and Nephrology0 (SwePub)302142 hsv//eng
653 a Erectile Dysfunction
653 a Macrophage Markers
653 a Major Pelvic Ganglion
653 a Neuroinflammation
653 a Neuroprotective
653 a Peripheral Nerve Injury
7001 a Sopko, Nikolai A.u Johns Hopkins University4 aut
7001 a Hannan, Johanna L.u Johns Hopkins University4 aut
7001 a Reinhardt, Allison A.u Ripon College4 aut
7001 a Kates, Maxu Johns Hopkins University4 aut
7001 a Yoshida, Takahirou Johns Hopkins University4 aut
7001 a Liu, Xiaopuu Johns Hopkins University4 aut
7001 a Castiglione, Fabiou Catholic University of Leuven4 aut
7001 a Hedlund, Petteru Lund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)kfar-phe
7001 a Weyne, Emmanuelu Catholic University of Leuven4 aut
7001 a Albersen, Maartenu Catholic University of Leuven4 aut
7001 a Bivalacqua, Trinity J.u Johns Hopkins University4 aut
7102 a Johns Hopkins Universityb Doai Memorial Hospital4 org
7730 t Journal of Sexual Medicined : Oxford University Press (OUP)g 14:2, s. 187-195q 14:2<187-195x 1743-6095x 1743-6109
8564 u http://dx.doi.org/10.1016/j.jsxm.2016.12.012y FULLTEXT
8564 u https://europepmc.org/articles/pmc5298795?pdf=render
8564 8u https://lup.lub.lu.se/record/869f46a7-e753-46b8-9762-cddb79cee11e
8564 8u https://doi.org/10.1016/j.jsxm.2016.12.012

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