id:"swepub:oai:lup.lub.lu.se:989abb38-0f7a-4ee6-8f60-c6d422042f63"
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03527naa a2200301 4500 | |
| 001 | oai:lup.lub.lu.se:989abb38-0f7a-4ee6-8f60-c6d422042f63 | |
| 003 | SwePub | |
| 008 | 160401s2002 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://lup.lub.lu.se/record/3321552 URI |
| 024 | 7 | a https://doi.org/10.1021/bp02029232 DOI |
| 040 | a (SwePub)lu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a art2 swepub-publicationtype |
| 072 | 7 | a ref2 swepub-contenttype |
| 100 | 1 | a Dainiak, Mariau Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biot-mda |
| 245 | 1 0 | a Polyelectrolyte-coated ion exchangers for cell-resistant expanded bed adsorption |
| 264 | 1 | b Wiley,c 2002 |
| 520 | a Adsorption chromatography in expanded beds is a widely used technology for direct capture of target proteins from fermentation broths. However, in many cases this method cannot be applied as a result of the strong tendency of cells or cell debris to interact with the adsorbent beads. To prevent contamination of the expanded bed with the biomass, STREAMLINE DEAE, anion exchanger designed for expanded bed adsorption, was modified with a layer of poly(acrylic acid) (PAA). The shielding layer of polyelectrolyte was attached to the surface of the matrix beads via electrostatic interactions. PAA with a high degree of polymerization was chosen to prevent diffusion of large polymer molecules into the pores of adsorbent. Thus, the shielding layer of PAA was adsorbed, only at the mouth of the pores of STREAMLINE DEAE beads and only marginally decreased the binding capacity of the ion exchanger for bovine serum albumin, the model protein in this study. PAA-coated STREAMLINE DEAE practically did not interact with yeast cells, which other-wise bound,strongly to the native adsorbent at neutral conditions. Cell-resistant PAA-coated anion exchanger was successfully used for isolation of BSA from the model protein mixture containing BSA, lysozyme (positively charged at applied conditions), and yeast cells. The layer of PAA was stable under mild elution conditions, and the modified adsorbent could be used in the repeated purification cycles. | |
| 650 | 7 | a TEKNIK OCH TEKNOLOGIERx Industriell bioteknik0 (SwePub)2092 hsv//swe |
| 650 | 7 | a ENGINEERING AND TECHNOLOGYx Industrial Biotechnology0 (SwePub)2092 hsv//eng |
| 700 | 1 | a Galaev, Igoru Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biot-iga |
| 700 | 1 | a Mattiasson, Bou Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biot-bma |
| 710 | 2 | a Bioteknikb Centrum för tillämpade biovetenskaper4 org |
| 773 | 0 | t Biotechnology Progressd : Wileyg 18:4, s. 815-820q 18:4<815-820x 1520-6033x 8756-7938 |
| 856 | 4 | u http://dx.doi.org/10.1021/bp0202923y FULLTEXT |
| 856 | 4 8 | u https://lup.lub.lu.se/record/332155 |
| 856 | 4 8 | u https://doi.org/10.1021/bp0202923 |
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