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PARP14 is a PARP wi...
PARP14 is a PARP with both ADP-ribosyl transferase and hydrolase activities
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- Đukić, Nina (author)
- University of Oxford
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- Strømland, Øyvind (author)
- University of Bergen,University of Oxford
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- Elsborg, Jonas Damgaard (author)
- University of Copenhagen
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- Munnur, Deeksha (author)
- University of Oxford
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- Zhu, Kang (author)
- University of Oxford
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- Schuller, Marion (author)
- University of Oxford
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- Chatrin, Chatrin (author)
- University of Oxford
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- Kar, Pulak (author)
- University of Oxford
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- Duma, Lena (author)
- University of Oxford
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- Suyari, Osamu (author)
- University of Oxford
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- Rack, Johannes Gregor Matthias (author)
- University of Exeter,University of Oxford
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- Baretić, Domagoj (author)
- University of Oxford
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- Crudgington, Dorian Richard Kenneth (author)
- University of Oxford
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- Groslambert, Joséphine (author)
- University of Oxford
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- Fowler, Gerissa (author)
- University of Oxford
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- Wijngaarden, Sven (author)
- Leiden University
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- Prokhorova, Evgeniia (author)
- University of Oxford
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- Rehwinkel, Jan (author)
- University of Oxford
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- Schüler, Herwig (author)
- Lund University,Lunds universitet,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Filippov, Dmitri V (author)
- Leiden University
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- Sanyal, Sumana (author)
- University of Oxford
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- Ahel, Dragana (author)
- University of Oxford
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- Nielsen, Michael L (author)
- University of Copenhagen
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- Smith, Rebecca (author)
- University of Oxford
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- Ahel, Ivan (author)
- University of Oxford
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(creator_code:org_t)
- 2023
- 2023
- English 18 s.
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In: Science Advances. - 2375-2548. ; 9:37
- Related links:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Subject headings
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- PARP14 is a mono-ADP-ribosyl transferase involved in the control of immunity, transcription, and DNA replication stress management. However, little is known about the ADP-ribosylation activity of PARP14, including its substrate specificity or how PARP14-dependent ADP-ribosylation is reversed. We show that PARP14 is a dual-function enzyme with both ADP-ribosyl transferase and hydrolase activity acting on both protein and nucleic acid substrates. In particular, we show that the PARP14 macrodomain 1 is an active ADP-ribosyl hydrolase. We also demonstrate hydrolytic activity for the first macrodomain of PARP9. We reveal that expression of a PARP14 mutant with the inactivated macrodomain 1 results in a marked increase in mono(ADP-ribosyl)ation of proteins in human cells, including PARP14 itself and antiviral PARP13, and displays specific cellular phenotypes. Moreover, we demonstrate that the closely related hydrolytically active macrodomain of SARS2 Nsp3, Mac1, efficiently reverses PARP14 ADP-ribosylation in vitro and in cells, supporting the evolution of viral macrodomains to counteract PARP14-mediated antiviral response.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- Humans
- Transferases
- Poly(ADP-ribose) Polymerase Inhibitors
- COVID-19
- Antiviral Agents
- Hydrolases
- Poly(ADP-ribose) Polymerases/genetics
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Đukić, Nina
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Strømland, Øyvin ...
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Elsborg, Jonas D ...
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Munnur, Deeksha
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Zhu, Kang
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Schuller, Marion
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show more...
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Chatrin, Chatrin
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Kar, Pulak
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Duma, Lena
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Suyari, Osamu
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Rack, Johannes G ...
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Baretić, Domagoj
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Crudgington, Dor ...
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Groslambert, Jos ...
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Fowler, Gerissa
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Wijngaarden, Sve ...
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Prokhorova, Evge ...
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Rehwinkel, Jan
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Schüler, Herwig
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Filippov, Dmitri ...
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Sanyal, Sumana
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Ahel, Dragana
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Nielsen, Michael ...
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Smith, Rebecca
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Ahel, Ivan
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Medical Biotechn ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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Lund University