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History and results of the two inter-laboratory round robin endotoxin assay studies on cotton dust
- Chun, DTW (author)
- Bartlett, K (author)
- Gordon, T (author)
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- Jacobs, RR (author)
- Lewis, DM (author)
- Liesivuori, J (author)
- Michel, O (author)
- Milton, DK (author)
- Rylander, R (author)
- Thorne, PS (author)
- White, EM (author)
- Brown, ME (author)
- Gunn, VS (author)
- Wurtz, H (author)
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- (creator_code:org_t)
- 2006
- 2006
- English.
- In: American Journal of Industrial Medicine. - : Wiley. - 0271-3586 .- 1097-0274. ; 49:4, s. 301-306
- Conference paper (peer-reviewed)
Abstract
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- Background In the US cotton industry, airborne cotton dust levels are regulated, and other countries are moving to specify safety limits for airborne endotoxins. There is concern about potential respiratory health hazards associated with agricultural and otherorganic dusts. In laboratories, ranking which samples have high and low levels of endotoxin is usually in good agreement between laboratories. When different laboratories assay identical samples, the levels differ The objective of this research was to evaluate the intra- and inter-laboratory variability for 13 laboratories measuring endotoxin in cotton dust. Method Two inter-laboratory round robin endotoxin assay studies were conducted using cotton dust. In the first round robin, each laboratory used their normal in-house assay method and then used a common extraction protocol. In the second round robin, a common extraction protocol and endotoxin assay, kit was used. Results The inter-laboratory, results using a common extraction protocol showed reduced differences. Using the same extraction protocol and endotoxin assay kit, the intra-laboratory variation was small and inter-laboratory variation was reduced but not enough for inter-laboratory agreement. Most of the laboratories were able to discern between the high and low endotoxin concentration dusts. Conclusions Standardization has reduced the differences in results between laboratories and possibly further standardization may bring closer inter-laboratory agreement.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- endotoxin assay
- limulus amoebocyte lysate test (LAL)
- study
- cotton dust
- round robin endotoxin assay
- inter-laboratory endotoxin assay study
- intra-laboratory endotoxin assay study
- lipopolysaccharides (LPS)
Publication and Content Type
- kon (subject category)
- ref (subject category)
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- Chun, DTW
- Bartlett, K
- Gordon, T
- Jacobs, RR
- Larsson, BM
- Larsson, Lennart
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- Lewis, DM
- Liesivuori, J
- Michel, O
- Milton, DK
- Rylander, R
- Thorne, PS
- White, EM
- Brown, ME
- Gunn, VS
- Wurtz, H
- show less...
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- MEDICAL AND HEALTH SCIENCES
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- Lund University
- Karolinska Institutet
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