id:"swepub:oai:lup.lub.lu.se:a7a97393-1746-4f5e-8595-1afa32b45e60"
Search: id:"swepub:oai:lup.lub.lu.se:a7a97393-1746-4f5e-8595-1afa32b45e60" > Glucose transport a...
- 1 of 1
- Previous record
- Next record
- To hitlist
Glucose transport activity measured in giant vesicles
-
- Hansen, Jesper S. (author)
- Lund University,Lunds universitet,Medicinsk strukturbiologi,Forskargrupper vid Lunds universitet,Medical Structural Biology,Lund University Research Groups
-
- Lindkvist-Petersson, Karin (author)
- Lund University,Lunds universitet,Medicinsk strukturbiologi,Forskargrupper vid Lunds universitet,Medical Structural Biology,Lund University Research Groups
- (creator_code:org_t)
- 2017-12-08
- 2018
- English 15 s.
- In: Methods in Molecular Biology. - New York, NY : Springer New York. - 1064-3745. ; 1713, s. 77-91
- Book chapter (peer-reviewed)
Abstract
Subject headings
Close
- Incorporation of membrane proteins and internal reporter systems directly into giant vesicles, during their formation from a hydrogel surface, has emerged as a promising new concept in membrane protein characterization. Here, we provide the detailed protocol for a glucose transporter activity assay based on giant vesicles containing a fluorescent enzyme-linked reporter system internally. This assay is applicable for the functional analysis of a variety of hexose-transporting proteins. We furthermore believe that it can aid in the development of drugs targeting hexose transporters.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Activity assay
- Fluorescence microscopy
- Giant vesicles
- Glucose transport
- Glucose transporters
- GLUT1
- Membrane protein
- Resorufin
Publication and Content Type
- kap (subject category)
- ref (subject category)
Find in a library
- Methods in Molecular Biology (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Hansen, Jesper S ...
- Lindkvist-Peters ...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Cell and Molecul ...
- Articles in the publication
- Methods in Molec ...
- By the university
- Lund University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
