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Development of an a...
Development of an amperometric biosensor based on acetylcholine esterase covalently bound to a new support material
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Khayyami, M (author)
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Pérez Pita, M T (author)
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Peña Garcia, N (author)
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- Johansson, Gillis (author)
- Lund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Danielsson, Bengt (author)
- Lund University,Lunds universitet,Tillämpad biokemi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Pure and Applied Biochemistry,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Larsson, Per-Olof (author)
- Lund University,Lunds universitet,Tillämpad biokemi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Pure and Applied Biochemistry,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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(creator_code:org_t)
- 1998
- 1998
- English.
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In: Talanta. - 1873-3573. ; 45:3, s. 557-563
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http://dx.doi.org/10...
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https://doi.org/10.1...
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Abstract
Subject headings
Close
- A new type of amperometric biosensor based on immobilised acetylcholine esterase was designed and constructed. The enzyme was immobilised on a flow-through working electrode, which was prepared from reticulated vitreous carbon (RVC) or from a composite material consisting of RVC and superporous agarose. The sensor was operated in FIA mode using acetylthiocholine as a substrate. The sensor responded to inhibitors such as paraoxon—10−9 mol was detected by the sensor in a non-optimised configuration. The practical lifetime of the sensor was at least 1 month.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Materialteknik -- Kompositmaterial och -teknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Materials Engineering -- Composite Science and Engineering (hsv//eng)
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Keyword
- Acetylthiocholine
- Acetylcholine esterase
- Biosensor
- RVC-electrode
- Amperometric
- Thiocholine
- Mediator
- Superporous agarose gel
Publication and Content Type
- art (subject category)
- ref (subject category)
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