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Identification of t...
Identification of the ligand of Pru p 3, a peach LTP
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- Cubells-Baeza, Nuria (author)
- Technical University of Madrid,Centre for Plant Biotechnology and Genomics
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- Gómez-Casado, Cristina (author)
- Lund University,Lunds universitet,Slemhinnans immunologi,Forskargrupper vid Lunds universitet,Mucosal Immunology,Lund University Research Groups
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- Tordesillas, Leticia (author)
- Icahn School of Medicine at Mount Sinai
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- Ramírez-Castillejo, Carmen (author)
- Centre for Plant Biotechnology and Genomics,Technical University of Madrid
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- Garrido-Arandia, María (author)
- Centre for Plant Biotechnology and Genomics,Technical University of Madrid
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- González-Melendi, Pablo (author)
- Technical University of Madrid
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- Herrero, María (author)
- CSIC - Estacion Experimental de Aula Dei EEAD
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- Pacios, Luis F. (author)
- Centre for Plant Biotechnology and Genomics,Technical University of Madrid
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- Díaz-Perales, Araceli (author)
- Centre for Plant Biotechnology and Genomics,Technical University of Madrid
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(creator_code:org_t)
- 2017-03-15
- 2017
- English 12 s.
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In: Plant Molecular Biology. - : Springer Science and Business Media LLC. - 0167-4412 .- 1573-5028. ; 94:1-2, s. 33-44
- Related links:
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http://dx.doi.org/10...
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http://oa.upm.es/500...
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Abstract
Subject headings
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- Key message: Pru p 3, a peach LTP, is located in pollinated flower styles and secreting downy hairs, transporting a derivative of camptothecin bound to phytosphingosine. Pru p 3 may inhibit a second pollination and may keep away herbivores until seed maturation. Abstract: The allergen Pru p 3, a peach lipid transfer protein, has been well studied. However, its physiological function remains to be elucidated. Our results showed that Pru p 3 usually carries a lipid ligand that play an essential role in its function in plants. Using ESI-qToF, we observed that the ligand was a derivative of camptothecin binding to phytosphingosine, wich that is inserted into the hydrophobic tunnel of the protein. In addition, the described ligand displayed topoisomerase I activity inhibition and self-fluorescence, both recognized as camptothecin properties. During flower development, the highest expression of Pru p 3 was detected in the styles of pollinated flowers, in contrast to its non-expression in unpollinated pistils, where expression decreased after anthesis. During ripening, the expression of Pru p 3 were observed mainly in peel but not in pulp. In this sense, Pru p 3 protein was also localized in trichomes covering the fruit epidermis.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Botanik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Botany (hsv//eng)
Keyword
- Camptothecin
- Flower development
- Fruit development
- Lipid transfer protein
- Pollination
- Pru p 3
- Secondary metabolites
Publication and Content Type
- art (subject category)
- ref (subject category)
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