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Plasmid deficiency ...
Plasmid deficiency in urogenital isolates of Chlamydia trachomatis reduces infectivity and virulence in a mouse model
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- Sigar, Ira M. (author)
- Dept Microbiol & Immunol, Chicago Coll Osteopath Med, Midwestern Univ, Downers Grove IL, USA
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- Schripsema, Justin H. (author)
- Dept Microbiol & Immunol, Chicago Coll Osteopath Med, Midwestern Univ, Downers Grove IL, USA
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- Wang, Yibing (author)
- Dept Clin & Expt Sci, Mol Microbiol Grp, Fac Med, Univ Southampton, Southampton, England
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- Clarke, Ian N. (author)
- Dept Clin & Expt Sci, Mol Microbiol Grp, Fac Med, Univ Southampton, Southampton, England
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- Cutcliffe, Lesley T. (author)
- Dept Clin & Expt Sci, Mol Microbiol Grp, Fac Med, Univ Southampton, Southampton, England
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- Seth-Smith, Helena M. B. (author)
- Wellcome Trust Sanger Inst, Hinxton, England
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- Thomson, Nicholas R. (author)
- Wellcome Trust Sanger Inst, Hinxton, England
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- Bjartling, Carina (author)
- Lund University,Lunds universitet,Urogynekologi och reproduktionsfarmakologi,Forskargrupper vid Lunds universitet,Urogynaecology and Reproductive Pharmacology,Lund University Research Groups,Dept Obstet & Gynecol, Malmö Univ Hosp, Malmö, Sweden
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- Unemo, Magnus (author)
- Region Örebro län,Dept Lab Med, Örebro University Hospital, Örebro, Sweden
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- Persson, Kenneth (author)
- Lund University,Lunds universitet,Klinisk mikrobiologi, Malmö,Forskargrupper vid Lunds universitet,Clinical Microbiology, Malmö,Lund University Research Groups,Dept Lab Med, Malmö Univ Hosp, Malmö, Sweden
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- Ramsey, Kyle H. (author)
- Dept Microbiol & Immunol, Chicago Coll Osteopath Med, Midwestern Univ, Downers Grove IL, USA
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(creator_code:org_t)
- Wiley-Blackwell, 2014
- 2014
- English.
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In: Pathogens and Disease. - : Wiley-Blackwell. - 2049-632X. ; 70:1, s. 61-69
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Abstract
Subject headings
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- We hypothesized that the plasmid of urogenital isolates of Chlamydia trachomatis would modulate infectivity and virulence in a mouse model. To test this hypothesis, we infected female mice in the respiratory or urogenital tract with graded doses of a human urogenital isolate of C.trachomatis, serovar F, possessing the cognate plasmid. For comparison, we inoculated mice with a plasmid-free serovar F isolate. Following urogenital inoculation, the plasmid-free isolate displayed significantly reduced infectivity compared with the wild-type strain with the latter yielding a 17-fold lower infectious dose to yield 50% infection. When inoculated via the respiratory tract, the plasmid-free isolate exhibited reduced infectivity and virulence (as measured by weight change) when compared to the wild-type isolate. Further, differences in infectivity, but not in virulence were observed in a C.trachomatis, serovar E isolate with a deletion within the plasmid coding sequence 1 when compared to a serovar E isolate with no mutations in the plasmid. We conclude that plasmid loss reduces virulence and infectivity in this mouse model. These findings further support a role for the chlamydial plasmid in infectivity and virulence in vivo.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Keyword
- Chlamydia
- plasmid
- mouse
- virulence
- infection
- genome
- Chlamydia
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Sigar, Ira M.
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Schripsema, Just ...
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Wang, Yibing
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Clarke, Ian N.
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Cutcliffe, Lesle ...
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Seth-Smith, Hele ...
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show more...
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Thomson, Nichola ...
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Bjartling, Carin ...
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Unemo, Magnus
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Persson, Kenneth
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Ramsey, Kyle H.
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Microbiology in ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Infectious Medic ...
- Articles in the publication
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Pathogens and Di ...
- By the university
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Lund University
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Örebro University