Improved chip design for integrated solid-phase microextraction in on-line proteomic sample preparation

Bergkvist, Jonas (author): Lund University,Lunds universitet,Institutioner vid LTH,Lunds Tekniska Högskola,Departments at LTH,Faculty of Engineering, LTH

Ekström, Simon (author): Lund University,Lunds universitet,Institutioner vid LTH,Lunds Tekniska Högskola,Departments at LTH,Faculty of Engineering, LTH

Wallman, Lars (author): Lund University,Lunds universitet,Institutioner vid LTH,Lunds Tekniska Högskola,Departments at LTH,Faculty of Engineering, LTH

Löfgren, Mikael (author)

Marko-Varga, György (author): Lund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH

Nilsson, Johan (author): Lund University,Lunds universitet,Institutioner vid LTH,Lunds Tekniska Högskola,Departments at LTH,Faculty of Engineering, LTH

Laurell, Thomas (author): Lund University,Lunds universitet,Institutioner vid LTH,Lunds Tekniska Högskola,Departments at LTH,Faculty of Engineering, LTH

(creator_code:org_t)

Related links:: http://dx.doi.org/10...; show more...; https://lup.lub.lu.s...; https://doi.org/10.1...; show less...

Abstract Subject headings

A recently introduced silicon microextraction chip (SMEC), used for on-line proteomic sample preparation, has proved to facilitate the process of protein identification by sample clean up and enrichment of peptides. It is demonstrated that a novel grid-SMEC design improves the operating characteristics for solid-phase microextraction, by reducing dispersion effects and thereby improving the sample preparation conditions. The structures investigated in this paper are treated both numerically and experimentally. The numerical approach is based on finite element analysis of the micro-fluidic flow in the microchip. The analysis is accomplished by use of the computational fluid dynamics-module FLOTRAN in the ANSYS(R) software package. The modeling and analysis of the previously reported weir-SMEC design indicates some severe drawbacks, that can be reduced by changing the microextraction chip geometry to the grid-SMEC design. The overall analytical performance was thereby improved and also verified by experimental work. Matrix-assisted laser desorption/ionization mass spectra of model peptides extracted from both the weir-SMEC and the new grid-SMEC support the numerical analysis results. Further use of numerical modeling and analysis of the SMEC structures is also discussed and suggested in this work.

By the author/editor: Bergkvist, Jonas; Ekström, Simon; Wallman, Lars; Löfgren, Mikael; Marko-Varga, Gyö ...; Nilsson, Johan; show more...; Laurell, Thomas; show less...

About the subject

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