Hormone-sensitive lipase is not required for cholesteryl ester hydrolysis in macrophages.

• Storage of cholesteryl esters in the cytoplasm of macrophages is one of the earliest and most ubiquitous event observed in the development of arteriosclerosis. Macrophages have an enormous capacity to uptake and store cholesterol in the form of cytosolic cholesteryl ester droplets. These stores are mobilized by the action of a neutral cholesteryl ester hydrolase (NCEH), producing free cholesterol that is either secreted to extracellular acceptors or reesterified. It has been proposed that hormone-sensitive lipase (HSL) is responsible for the NCEH activity in macrophages. The present work shows, however, that peritoneal macrophages from HSL null mice hydrolyze cytosolic stores of cholesteryl esters at a comparable rate to that of peritoneal macrophages from wild-type mice, therefore demonstrating that HSL is not the main NCEH in macrophages.

Ämnesord

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Nyckelord

Cholesterol Esterase : metabolism

Cholesterol Esterase : genetics

Cholesterol Esterase : deficiency

Cultured

Cells

Bucladesine : pharmacology

Western

Blotting

Arteriosclerosis : metabolism

Animal

Adipose Tissue : enzymology

Peritoneal : metabolism

Mice

Inbred Strains

Knockout

Cholesterol Esters : metabolism

Cytoplasm : metabolism

Hydrolysis

Macrophages

Peritoneal : drug effects

Publikations- och innehållstyp

art (ämneskategori)

ref (ämneskategori)