Search: id:"swepub:oai:lup.lub.lu.se:e53aea91-28dd-4e6c-b5fb-350eccb23a72" >
Antifungal activiti...
Antifungal activities of secondary metabolites isolated from liquid fermentations of Stereum hirsutum (Sh134-11) against Botrytis cinerea (grey mould agent)
-
- Aqueveque, Pedro (author)
- University of Concepción
-
Céspedes, Carlos L. (author)
-
- Becerra, José (author)
- University of Concepción
-
show more...
-
- Aranda, Mario (author)
- University of Concepción
-
- Sterner, Olov (author)
- Lund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
-
show less...
-
(creator_code:org_t)
- Elsevier BV, 2017
- 2017
- English.
-
In: Food and Chemical Toxicology. - : Elsevier BV. - 0278-6915. ; 109, s. 1048-1054
- Related links:
-
http://dx.doi.org/10...
-
show more...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Extracts obtained from liquid mycelial fermentations of the Chilean fungus Stereum hirsutum (Sh134-11) showed antifungal activity against Botrytis cinerea. Two types of extracts were obtained: EtOAc-extract (liquid phase) and MeOH-extract (mycelial phase). Plate diffusion assay showed that EtOAc-extracts were more active than MeOH-extracts. A large-scale fermentation of Sh134-11 and chromatographic methods allowed to isolated four compounds: MS-3, Vibralactone, Vibralactone B and Sterenin D. Only Sterenin D showed antifungal activity against B. cinerea in the tests performed. Effects on the mycelial growth of B. cinerea showed that Sterenin D showed inhibition at 1000-2000 μg/mL reaching 67% and 76% respectively. Sterenin D was more effective to control the sporogenesis, inhibiting in 96% the sporulation at 500 μg/mL. Assays showed that Sterenin D exhibited a minimal fungicidal concentration (MFC) of 50 μg/mL and minimal inhibitory concentration (MIC) at 20 μg/mL.Our study indicated that submerged fermentations of Chilean S. hirsutum (Sh134-11) produced extracts with antifungal activity and Sterenin D is responsible for this activity, which could be used as possible biofungicides alternative to synthetic fungicides.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Keyword
- Antifungal
- Botrytis
- Chilean fungi
- Sterenin D
- Stereum
- Submerged cultures
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database