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Stimulation of χ transcription by a decamer‐dependent, synergistic mechanism
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- Sigvardsson, Mikael (author)
- Lund University,Lunds universitet,Immunologi,Forskargrupper vid Lunds universitet,Immunology,Lund University Research Groups
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- Bemark, Mats (author)
- Lund University,Lunds universitet,Immunologi,Forskargrupper vid Lunds universitet,Immunology,Lund University Research Groups
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- Leanderson, Tomas (author)
- Lund University,Lunds universitet,Immunologi,Forskargrupper vid Lunds universitet,Immunology,Lund University Research Groups
- (creator_code:org_t)
- Wiley, 1995
- 1995
- English.
- In: European Journal of Immunology. - : Wiley. - 0014-2980 .- 1521-4141. ; 25:1, s. 298-301
- Journal article (peer-reviewed)
Abstract
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- The intact SP6 χ promoter stimulated transcription 30 times more efficiently than did a control promoter consisting of a TATA motif as the only promoter element. Mutation of the SP6 χ promoter decamer in two positions reduced the transcriptional stimulation activity by over 90%. Promoters containing the SP6 χ promoter octamer or a consensus octamer in front of a TATA box were ineffective immunoglobulin promoters and stimulated at the most 15% of maximal transcription. Identical results were obtained after transfection of untransformed mouse splenic B cells stimulated by lipopolysaccharide, that express high levels of Oct2A, or of S194 cells that express negligible levels of Oct2A. Selective mutations in the penta‐decamer (pd), χY or early B cell factor (EBF) elements of the promoter reduced transcriptional stimulation by 20–30% in untransformed B cells. In S194 plasmacytoma cells the EBF mutation was functionally silent while the χY and pd mutations reduced transcriptional activation by 60‐70% in this cell line. A mutation in a TATA‐proximal E‐box motif did not alter the functional activity of the promoter in either cell population. It can be concluded that χ promoter function is highly dependent on complex interactions between individual promoter elements and that the decamer motif is pivotal for these interactions. The relative functional activity of a given promoter varied according to the target cell population used for the functional assay.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- Oct proteins
- Transcription
- χ‐promoter
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- art (subject category)
- ref (subject category)
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