Sökning: id:"swepub:oai:lup.lub.lu.se:fe5f6f18-4787-47ab-9d70-4681c57781c2" >
Relationship Betwee...
Relationship Between Serum Response Factor and Androgen Receptor in Prostate Cancer
-
Prencipe, Maria (författare)
-
O'Neill, Amanda (författare)
-
O'Hurley, Gillian (författare)
-
visa fler...
-
Nguyen, Lan K. (författare)
-
Fabre, Aurelie (författare)
-
- Bjartell, Anders (författare)
- Lund University,Lunds universitet,Urologisk cancerforskning, Malmö,Forskargrupper vid Lunds universitet,Urological cancer, Malmö,Lund University Research Groups
-
Gallagher, William M. (författare)
-
Morrissey, Colm (författare)
-
Kay, Elaine W. (författare)
-
Watson, R. William (författare)
-
visa färre...
-
(creator_code:org_t)
- 2015-08-07
- 2015
- Engelska.
-
Ingår i: The Prostate. - : Wiley. - 0270-4137. ; 75:15, s. 1704-1717
- Relaterad länk:
-
http://dx.doi.org/10...
-
visa fler...
-
https://europepmc.or...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- BACKGROUND. Serum response factor (SRF) is an important transcription factor in castrate-resistant prostate cancer (CRPC). Since CRPC is associated with androgen receptor (AR) hypersensitivity, we investigated the relationship between SRF and AR. MATERIALS AND METHODS. Transcriptional activity was assessed by luciferase assay. Cell proliferation was measured by MTT and flow cytometry. Protein expression in patients was assessed by immunohistochemistry. RESULTS. To investigate AR involvement in SRF response to androgen, AR expression was down-regulated using siRNA. This resulted in the abrogation of SRF induction post-DHT. Moreover, DHT stimulation failed to induce SRF transcriptional activity in AR-negative PC346 DCC cells, which was only restored following AR over-expression. Next, SRF expression was down-regulated by siRNA, resulting in AR increased transcriptional activity in castrate-resistant LNCaP Abl cells but not in the parental LNCaP. This negative feedback loop in the resistant cells was confirmed by immunohistochemistry which showed a negative correlation between AR and SRF expression in CRPC bone metastases and a positive correlation in androgen-naive prostatectomies. Cell proliferation was next assessed following SRF inhibition, demonstrating that SRF inhibition is more effective than AR inhibition in castrate-resistant cells. CONCLUSION. Our data support SRF as a promising therapeutic target in combination with current treatments. (C) 2015 Wiley Periodicals, Inc.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- castrate-resistant prostate cancer
- serum response factor
- androgen
- receptor
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Prencipe, Maria
-
O'Neill, Amanda
-
O'Hurley, Gillia ...
-
Nguyen, Lan K.
-
Fabre, Aurelie
-
Bjartell, Anders
-
visa fler...
-
Gallagher, Willi ...
-
Morrissey, Colm
-
Kay, Elaine W.
-
Watson, R. Willi ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Cancer och onkol ...
- Artiklar i publikationen
-
The Prostate
- Av lärosätet
-
Lunds universitet