A genome-wide screening uncovers the role of CCAR2 as an antagonist of DNA end resection

Lopez-Saavedra, A (author)

Gomez-Cabello, D (author)

Dominguez-Sanchez, MS (author)

Mejias-Navarro, F (author)

Fernandez-Avila, MJ (author)

Dinant, C (author)

Martinez-Macias, MI (author)

Huertas, P (author)

(creator_code:org_t)

2016-08-09
2016
English.
In: Nature communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 7, s. 12364-

Related links:: https://www.nature.c...; show more...; http://kipublication...; https://doi.org/10.1...; show less...

Abstract Subject headings

There are two major and alternative pathways to repair DNA double-strand breaks: non-homologous end-joining and homologous recombination. Here we identify and characterize novel factors involved in choosing between these pathways; in this study we took advantage of the SeeSaw Reporter, in which the repair of double-strand breaks by homology-independent or -dependent mechanisms is distinguished by the accumulation of green or red fluorescence, respectively. Using a genome-wide human esiRNA (endoribonuclease-prepared siRNA) library, we isolate genes that control the recombination/end-joining ratio. Here we report that two distinct sets of genes are involved in the control of the balance between NHEJ and HR: those that are required to facilitate recombination and those that favour NHEJ. This last category includes CCAR2/DBC1, which we show inhibits recombination by limiting the initiation and the extent of DNA end resection, thereby acting as an antagonist of CtIP.

By the author/editor: Lopez-Saavedra, ...; Gomez-Cabello, D; Dominguez-Sanche ...; Mejias-Navarro, ...; Fernandez-Avila, ...; Dinant, C; show more...; Martinez-Macias, ...; Bartek, J; Huertas, P; show less...

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