Clustering of conformational IgE epitopes on the major dog allergen Can f 1

Curin, M (author)

Weber, M (author)

Hofer, G (author)

Keller, W (author)

Reininger, R (author)

Swoboda, I (author)

Spitzauer, S (author)

Focke-Tejkl, M (author)

Valenta, R (author)

(creator_code:org_t)

2017-09-22
2017
English.
In: Scientific reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 7:1, s. 12135-

Related links:: https://www.nature.c...; show more...; http://kipublication...; https://doi.org/10.1...; show less...

Abstract Subject headings

Immunoglobulin E (IgE)-associated allergy affects more than 25% of the population. Can f 1 is the major dog allergen associated with respiratory symptoms but the epitopes recognized by allergic patients IgE on Can f 1 are unknown. To characterize IgE epitopes of Can f 1 recognized by dog allergic patients, six overlapping peptides spanning the Can f 1 sequence were synthesized. In direct IgE epitope mapping experiments peptides were analyzed for IgE reactivity by dot blot and Enzyme-linked immunosorbent assay (ELISA) with sera from dog allergic patients. For indirect epitope-mapping, rabbits were immunized with the peptides to generate specific IgG antibodies which were used to inhibit allergic patients’ IgE binding to Can f 1. IgE binding sites were visualized on a model of the Can f 1 three-dimensional structure. We found that Can f 1 does not contain any relevant sequential IgE epitopes. However, IgE inhibition experiments with anti-peptide specific IgGs showed that Can f 1 N- and C-terminal portion assembled a major conformational binding site. In conclusion, our study is the first to identify the major IgE epitope-containing area of the dog allergen Can f 1. This finding is important for the development of allergen-specific treatment strategies.

By the author/editor: Curin, M; Weber, M; Hofer, G; Apostolovic, D; Keller, W; Reininger, R; show more...; Swoboda, I; Spitzauer, S; Focke-Tejkl, M; van Hage, M; Valenta, R; show less...

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.