Knockdown of TRPC3 with siRNA coupled to carbon nanotubes results in decreased insulin-mediated glucose uptake in adult skeletal muscle cells

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Knockdown of TRPC3 with siRNA coupled to carbon nanotubes results in decreased insulin-mediated glucose uptake in adult skeletal muscle cells

Lanner, Johanna T. (author): Karolinska Institutet

Bruton, Joseph D. (author): Karolinska Institutet

Assefaw-Redda, Yohannes (author): KTH,Integrerade komponenter och kretsar

Andronache, Zoita (author)

Severa, Denise (author)

Zhang, Zhibin (author): KTH,Integrerade komponenter och kretsar

Melzer, Werner (author)

Zhang, Shi-Li (author): KTH,Integrerade komponenter och kretsar

Katz, Abram (author): Karolinska Institutet

Westerblad, Hakan (author): Karolinska Institutet

Zhang, SJ (author): Karolinska Institutet

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(creator_code:org_t)

2009-01-13
2009
English.
In: The FASEB Journal. - : Wiley. - 0892-6638 .- 1530-6860. ; 23:6, s. 1728-1738

Related links:: https://urn.kb.se/re...; show more...; https://doi.org/10.1...; http://kipublication...; show less...

Journal article (peer-reviewed)

Abstract Subject headings

The involvement of Ca2+ in the insulin-mediated signaling cascade, resulting in glucose uptake in skeletal muscle, is uncertain. Here, we test the hypothesis that Ca2+ influx through canonical transient receptor potential 3 (TRPC3) channels modulates insulin-mediated glucose uptake in adult skeletal muscle. Experiments were performed on adult skeletal muscle cells of wild-type (WT) and obese, insulin-resistant ob/ob mice. Application of the diacylglycerol analog 1-oleyl-2-acetyl-sn-glycerol (OAG) induced a nonselective cation current, which was inhibited by the addition of anti-TRPC3 antibody in the patch pipette and smaller in ob/ob than in WT cells. Knockdown of TRPC3, using a novel technique based on small interfering RNA (siRNA) coupled to functionalized carbon nanotubes, resulted in pronounced (similar to 70%) decreases in OAG-induced Ca2+ influx and insulin-mediated glucose uptake. TRPC3 and the insulin-sensitive glucose transporter 4 (GLUT4) coimmunoprecipitated, and immunofluorescence staining showed that they were colocalized in the proximity of the transverse tubular system, which is the predominant site of insulin-mediated glucose transport in skeletal muscle. In conclusion, our results indicate that TRPC3 interacts functionally and physically with GLUT4, and Ca2+ influx through TRPC3 modulates insulin-mediated glucose uptake. Thus, TRPC3 is a potential target for treatment of insulin-resistant conditions.-Lanner, J. T., Bruton, J. D., Assefaw-Redda, Y., Andronache, Z., Zhang, S.- J., Severa, D., Zhang, Z.- B., Melzer, W., Zhang, S.-L., Katz, A., Westerblad, H. Knockdown of TRPC3 with siRNA coupled to carbon nanotubes results in decreased insulin-mediated glucose uptake in adult skeletal muscle cells. FASEB J. 23, 1728-1738 (2009)

Keyword

Ca2
sarcolemma
transverse tubules
near-membrane Ca2
colocalization
protein-kinase-c
stimulated glut4 translocation
cation channels
transverse tubules
plasma-membrane
calcium sensor
vesicle fusion
slow-twitch
ca2+ influx
activation

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ref (subject category)
art (subject category)

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By the author/editor: Lanner, Johanna ...; Bruton, Joseph D ...; Assefaw-Redda, Y ...; Andronache, Zoit ...; Severa, Denise; Zhang, Zhibin; show more...; Melzer, Werner; Zhang, Shi-Li; Katz, Abram; Westerblad, Haka ...; Zhang, SJ; show less...

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