Search: id:"swepub:oai:prod.swepub.kib.ki.se:1934237" >
Parallel changes in...
Parallel changes in nuclear and cytosolic calcium in mouse pancreatic beta-cells
-
Brown, GR (author)
-
- Kohler, M (author)
- Karolinska Institutet
-
- Berggren, PO (author)
- Karolinska Institutet
-
(creator_code:org_t)
- Portland Press Ltd. 1997
- 1997
- English.
-
In: The Biochemical journal. - : Portland Press Ltd.. - 0264-6021 .- 1470-8728. ; 325325 ( Pt 3), s. 771-778
- Related links:
-
https://europepmc.or...
-
show more...
-
http://kipublication...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- In the neuroendocrine pancreatic β-cell, elevations in intracellular Ca2+ lead to insulin secretion and the initiation of gene transcription. However, the relationship between cytosolic and nuclear Ca2+ in these cells is unknown. The Ca2+ permeability of the nuclear membrane would therefore determine if Ca2+ could play a direct role in Ca2+-dependent nuclear processes. Using confocal fluorescence microscopy with the ratiometric Ca2+ indicator indo-1 and carefully correcting for compartmentalized indicator, we now demonstrate that there is no difference between the nuclear Ca2+ concentration and the cytosolic Ca2+ concentration ([Ca2+]c) in the resting β-cell. Slow Ca2+ oscillations induced by glucose, fast oscillations induced by glucagon-like peptide-1 and responses to potassium and carbachol all indicate that changes in cytosolic Ca2+ are reflected within the nucleus. We conclude that there are no restrictions on Ca2+ entry into the nucleus of the pancreatic β-cell subsequent to increases in [Ca2+]c. This implies that any signal involved in increasing [Ca2+]c, and thereby insulin release, may also promote nuclear Ca2+-induced gene transcription.
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database