Search: id:"swepub:oai:prod.swepub.kib.ki.se:1938498" >
Human herpesvirus-8...
Human herpesvirus-8-encoded LNA-1 accumulates in heterochromatin- associated nuclear bodies
-
- Szekely, L (author)
- Karolinska Institutet
-
Kiss, C (author)
-
Mattsson, K (author)
-
show more...
-
- Kashuba, E (author)
- Karolinska Institutet
-
- Pokrovskaja, K (author)
- Karolinska Institutet
-
Juhasz, A (author)
-
Holmvall, P (author)
-
- Klein, G (author)
- Karolinska Institutet
-
show less...
-
(creator_code:org_t)
- Microbiology Society, 1999
- 1999
- English.
-
In: The Journal of general virology. - : Microbiology Society. - 0022-1317 .- 1465-2099. ; 8080 ( Pt 11), s. 2889-2900
- Related links:
-
https://doi.org/10.1...
-
show more...
-
http://kipublication...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Subnuclear distribution of the human herpesvirus-8 (HHV-8)- encoded nuclear protein LNA-1 was analysed at high resolution in body cavity (BC) lymphoma-derived cell lines, in cell hybrids between BC cells and various human and mouse cells and in freshly infected K562 and ECV cell lines. Three-dimensional reconstruction of nuclei from optical sections and quantitative analysis of the distribution of LNA-1 fluorescence in relation to chromatin showed that LNA-1 associates preferentially with the border of heterochromatin in the interphase nuclei. This was further confirmed in the following systems: in endo- and exonuclease-digested nuclei, in human–mouse (BC-1–Sp2- 0) hybrids and on chromatin spreads. LNA-1 was found to bind to mitotic chromosomes at random. Epstein–Barr virus (EBV), but not HHV-8, was rapidly lost from mouse–human hybrid cells in parallel with the loss of human chromosomes. HHV-8 could persist on the residual mouse background for more than 8 months. In early human–mouse hybrids that contain a single fused nucleus, LNA-1 preferentially associates with human chromatin. After the gradual loss of the human chromosomes, LNA-1 becomes associated with the murine pericentromeric heterochromatin. In human–human hybrids derived from the fusion of the HHV-8-carrying BCBL-1 cells and the EBV-immortalized lymphoblastoid cell line IB4, LNA-1 did not co-localize with EBNA-1, EBNA-2, EBNA-5 or EBNA-6. LNA-1 was not associated with PML containing ND10 bodies either. DNase but not RNase or detergent treatment of isolated nuclei destroys LNA-1 bodies. In advanced apoptotic cells LNA- 1 bodies remain intact but are not included in the apoptotic bodies themselves.
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database