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Amelotin--a Novel S...
Amelotin--a Novel Secreted, Ameloblast-specific Protein
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Iwasaki, K (author)
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Bajenova, E (author)
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Somogyi-Ganss, E (author)
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Miller, M (author)
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Nguyen, V (author)
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Nourkeyhani, H (author)
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Gao, Y (author)
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Wendel, M (author)
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Ganss, B (author)
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- 2016-12-15
- 2005
- English.
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In: Journal of dental research. - : SAGE Publications. - 0022-0345 .- 1544-0591. ; 84:12, s. 1127-1132
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http://kipublication...
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https://doi.org/10.1...
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Abstract
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- We aimed to analyze the differential gene expression in various murine dental tissues, expecting to find novel factors that are involved in tooth formation. We here describe the identification of a novel ameloblast-specific gene, amelotin (AMTN), by differential display polymerase chain-reaction (DD-PCR) analysis of microdissected ameloblasts, odontoblasts, dental pulp, and alveolar bone cells of 10-day-old mouse incisors. The conceptually translated protein sequence was unique and showed significant homology only with its human orthologue. The amelotin genes from mouse and human displayed a similar exon-intron structure and were expressed from loci on chromosomes 5 and 4, respectively, which have been associated with various forms of amelogenesis imperfecta. Expression of amelotin mRNA was restricted to maturation-stage ameloblasts in developing murine molars and incisors. Amelotin protein was efficiently secreted from transfected cells in culture. Taken together, our findings suggest that amelotin is a novel factor produced by ameloblasts that plays a critical role in the formation of dental enamel.
Publication and Content Type
- ref (subject category)
- art (subject category)
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