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Detecting Selective...
Detecting Selective Protein Binding Inside Plasmonic Nanopores: Toward a Mimic of the Nuclear Pore Complex
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- Malekian, Bita, 1986 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Schoch, Rafael L. (author)
- Universität Basel,University of Basel
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- Robson, Timothy, 1990 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Ferrand-Drake Del Castillo, Gustav, 1990 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Xiong, Kunli, 1987 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Emilsson, Gustav, 1989 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Kapinos, Larisa E. (author)
- Universität Basel,University of Basel
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- Lim, Roderick Y. H. (author)
- Universität Basel,University of Basel
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- Dahlin, Andreas, 1980 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- 2018-12-21
- 2018
- English.
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In: Frontiers in Chemistry. - : Frontiers Media SA. - 2296-2646. ; 6:December 2018
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Abstract
Subject headings
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- Biosensors based on plasmonic nanostructures offer label-free and real-time monitoring of biomolecular interactions. However, so do many other surface sensitive techniques with equal or better resolution in terms of surface coverage. Yet, plasmonic nanostructures offer unique possibilities to study effects associated with nanoscale geometry. In this work we use plasmonic nanopores with double gold films and detect binding of proteins inside them. By thiol and trietoxysilane chemistry, receptors are selectively positioned on the silicon nitride interior walls. Larger (similar to 150 nm) nanopores are used detect binding of averaged sized proteins (similar to 60 kg/mol) with high signal to noise (>100). Further, we fabricate pores that approach the size of the nuclear pore complex (diameter down to 50 nm) and graft disordered phenylalanine-glycine nucleoporin domains to the walls, followed by titration of karyopherin beta 1 transport receptors. The interactions are shown to occur with similar affinity as determined by conventional surface plasmon resonance on planar surfaces. Our work illustrates another unique application of plasmonic nanostructures, namely the possibility to mimic the geometry of a biological nanomachine with integrated optical sensing capabilities.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Läkemedelskemi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medicinal Chemistry (hsv//eng)
Keyword
- sensors
- nanopores
- nuclear pore complex
- proteins
- plasmons
Publication and Content Type
- art (subject category)
- ref (subject category)
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Malekian, Bita, ...
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Schoch, Rafael L ...
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Robson, Timothy, ...
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Ferrand-Drake De ...
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Xiong, Kunli, 19 ...
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Emilsson, Gustav ...
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show more...
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Kapinos, Larisa ...
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Lim, Roderick Y. ...
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Dahlin, Andreas, ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biophysics
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Medicinal Chemis ...
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Frontiers in Che ...
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Chalmers University of Technology