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Subcellular localisation of cholesterol and phosphocholine with pattern-recognition-imaging-TOF-SIMS
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- Malmberg, Per, 1974 (author)
- Göteborgs universitet,University of Gothenburg
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- Nygren, Håkan (author)
- Göteborgs universitet,University of Gothenburg
- Sjövall, Peter (author)
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- Lausmaa, Jukka (author)
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- (creator_code:org_t)
- 2004
- 2004
- English.
- In: Spectroscopy. ; 18:4, s. 503-512
- Journal article (peer-reviewed)
Abstract
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- Molecular ions of cholesterol, and its fragments, and phosphocholine fragments of phospholipids, were localized in single cells with a resolution of 7000 or high lateral resolution. High mass resolution spectra were recorded on reference samples of pure cholesterol and phosphatidylcholine. Characteristic fragment peaks and the silver cationised quasimolecular ion [M+Ag]+ were selected as a pattern for the identification of the lipids in TOF-SIMS images of surface-adhering leukocytes. The localisation of membrane lipids showed lateral heterogeneity over the cell surface.
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- Nygren, Håkan
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- Spectroscopy
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- Chalmers University of Technology
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