Visualization of β-carotene and starch granules in plant cells using CARS and SHG microscopy

Brackmann, Christian, 1973 (author): Chalmers tekniska högskola,Chalmers University of Technology

Bengtsson, Anton, 1979 (author): Chalmers tekniska högskola,Chalmers University of Technology

Alminger, Marie, 1957 (author): Chalmers tekniska högskola,Chalmers University of Technology

Svanberg, Ulf, 1945 (author): Chalmers tekniska högskola,Chalmers University of Technology

Enejder, Annika, 1969 (author): Chalmers tekniska högskola,Chalmers University of Technology

(creator_code:org_t)

2010-08-28
2010
English.
In: Journal of Raman Spectroscopy. - : Wiley. - 0377-0486 .- 1097-4555. ; 42:4, s. 586-592

Related links:: http://dx.doi.org/10...; show more...; https://doi.org/10.1...; https://research.cha...; show less...

Abstract Subject headings

Information on the content and bioavailability of provitamin A carotenoids, such as beta-carotene, in plant foods is of great interest due to the widespread vitamin A deficiency in developing countries. While the amount of beta-carotene can readily be quantified with analytical techniques, there is limited information on beta-carotene morphology in native plant materials. Here, we introduce nonlinear microscopy for three-dimensional, label-free imaging of carotenoids in fresh and thermally treated plant tissues, providing quantitative information at single-aggregate level and detailed insight into their distribution. Carotenoids in orange-fleshed sweet potato (OFSP), carrot, and mango were visualized by coherent anti-Stokes Raman scattering (CARS) microscopy and, in the case of OFSP, related to the plant-matrix morphology by simultaneous second-harmonic generation (SHG) microscopy of starch granules. Sizes, shapes, densities, and location of different types of carotenoid bodies were quantified. While OFSP and carrot showed heterogeneous rod-shaped bodies with high carotenoid densities indicated by higher CARS signals, the carotenoid-filled lipid droplets in mango appeared as homogeneous low-density aggregates of rounded shape. In addition, beta-carotene densities and morphologies in OFSP were studied after thermal processing, showing that the bodies remain intact despite significant changes of the surrounding starch granules.

By the author/editor: Brackmann, Chris ...; Bengtsson, Anton ...; Alminger, Marie, ...; Svanberg, Ulf, 1 ...; Enejder, Annika, ...

About the subject

AGRICULTURAL SCIENCES: AGRICULTURAL SCI ...; and Agriculture Fore ...; and Food Science

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