Improving heterologous protein secretion at aerobic conditions by activating hypoxia-induced genes in Saccharomyces cerevisiae

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Fältnamn	Indikatorer	Metadata
000		03091naa a2200397 4500
001		oai:research.chalmers.se:5a7635c7-cdb1-41be-bff5-e5a109fac214
003		SwePub
008		171007s2015 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://doi.org/10.1093/femsyr/fov0702 DOI
024	7	a https://research.chalmers.se/publication/2300202 URI
040		a (SwePub)cth
041		a engb eng
042		9 SwePub
072	7	a art2 swepub-publicationtype
072	7	a ref2 swepub-contenttype
100	1	a Liu, Lifang,d 1979u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)lifang
245	1 0	a Improving heterologous protein secretion at aerobic conditions by activating hypoxia-induced genes in Saccharomyces cerevisiae
264		c 2015-07-27
264	1	b Oxford University Press (OUP),c 2015
520		a Oxygen is important for normal aerobic metabolism, as well as for protein production where it is needed for oxidative protein folding. However, several studies have reported that anaerobic conditions seem to be more favorable in terms of recombinant protein production. We were interested in increasing recombinant protein production under aerobic conditions so we focused on Rox1p regulation. Rox1p is a transcriptional regulator, which in oxidative conditions represses genes induced in hypoxia. We deleted ROX1 and studied the effects on the production of recombinant proteins in Saccharomyces cerevisiae. Intriguingly, we found a 100% increase in the recombinant fungal alpha-amylase yield, as well as productivity. Varied levels of improvements were also observed for the productions of the human insulin precursor and the yeast endogenous enzyme invertase. Based on the genome-wide transcriptional response, we specifically focused on the effect of UPC2 upregulation on protein production and suggested a possible mechanistic explanation.
650	7	a NATURVETENSKAPx Biologix Mikrobiologi0 (SwePub)106062 hsv//swe
650	7	a NATURAL SCIENCESx Biological Sciencesx Microbiology0 (SwePub)106062 hsv//eng
653		a ROX1
653		a lipid classes
653		a alpha-amylase
653		a UPC2
700	1	a Zhang, Yiming,d 1986u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)yimingz
700	1	a Liu, Zihe,d 1984u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)zihe
700	1	a Petranovic Nielsen, Dina,d 1975u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)dinap
700	1	a Nielsen, Jens B,d 1962u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)nielsenj
710	2	a Chalmers tekniska högskola4 org
773	0	t FEMS Yeast Researchd : Oxford University Press (OUP)g 15:7, s. 10-q 15:7<10-x 1567-1356x 1567-1364
856	4	u http://dx.doi.org/10.1093/femsyr/fov070y FULLTEXT
856	4	u https://academic.oup.com/femsyr/article-pdf/15/7/fov070/16742128/fov070.pdf
856	4 8	u https://doi.org/10.1093/femsyr/fov070
856	4 8	u https://research.chalmers.se/publication/230020

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Av författaren/redakt...: Liu, Lifang, 197 ...; Zhang, Yiming, 1 ...; Liu, Zihe, 1984; Petranovic Niels ...; Nielsen, Jens B, ...

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