Image analysis in non-linear microscopy

Hagmar, Jonas, 1974 (author)

Brackmann, Christian, 1973 (author): Chalmers tekniska högskola,Chalmers University of Technology

Gustavsson, Tomas, 1954 (author): Chalmers tekniska högskola,Chalmers University of Technology

Enejder, Annika, 1969 (author): Chalmers tekniska högskola,Chalmers University of Technology

(creator_code:org_t)

Abstract Subject headings

The ability to automatically extract quantitative data from non-linear microscopy images is here explored, taking their non-linear character into account. Objects of different degree of complexity were investigated: theoretical images of spherical objects, experimentally collected Coherent Anti-Stokes Raman Scattering images of polystyrene spheres in background generating agar, well-separated lipid droplets in living yeast cells and conglomerations of lipid droplets in living C. elegans nematodes. The in linear microscopy useful measure of Full-Width-at-Half-Maximum (FWHM) was shown to provide inadequate measures of object size due the non-linear density dependence of the signal. Instead, the capability of four state-of-the-art image analysis algorithms was evaluated. Among these Local thresholding was found to be the widest applicable segmentation algorithm.

NATURVETENSKAP -- Biologi (hsv//swe)
NATURAL SCIENCES -- Biological Sciences (hsv//eng)
NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
NATURVETENSKAP -- Biologi -- Zoologi (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Zoology (hsv//eng)
NATURVETENSKAP -- Data- och informationsvetenskap -- Datorseende och robotik (hsv//swe)
NATURAL SCIENCES -- Computer and Information Sciences -- Computer Vision and Robotics (hsv//eng)

By the author/editor: Hagmar, Jonas, 1 ...; Brackmann, Chris ...; Gustavsson, Toma ...; Enejder, Annika, ...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Physical Science ...; and Atom and Molecul ...

NATURAL SCIENCES: NATURAL SCIENCES; and Physical Science ...; and Other Physics To ...

NATURAL SCIENCES: NATURAL SCIENCES; and Computer and Inf ...; and Computer Vision ...

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