id:"swepub:oai:research.chalmers.se:78758910-9b5c-49d0-acc2-3d6b211bfebe"
Search: id:"swepub:oai:research.chalmers.se:78758910-9b5c-49d0-acc2-3d6b211bfebe" > Assessment of the b...
- 1 of 1
- Previous record
- Next record
- To hitlist
Assessment of the biomass hydrolysis potential in bacterial isolates from a volcanic environment: Biosynthesis of the corresponding activities
-
- Stathopoulou, P.M. (author)
- University of Athens
-
- Galanopoulou, A.P. (author)
- University of Athens
-
- Anasontzis, George E, 1980 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
- show more...
- show less...
- (creator_code:org_t)
- 2012-06-20
- 2012
- English.
- In: World Journal of Microbiology and Biotechnology. - : Springer Science and Business Media LLC. - 1573-0972 .- 0959-3993. ; 28:9, s. 2889-2902
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- The biomass degrading enzymatic potential of 101 thermophilic bacterial strains isolated from a volcanic environment (Santorini, Aegean Sea, Greece) was assessed. 80 % of the strains showed xylanolytic activity in Congo Red plates, while only eight could simultaneously hydrolyze cellulose. Fifteen isolates were selected on the basis of their increased enzyme production, the majority of which was identified as Geobacilli through 16S rDNA analysis. In addition, the enzymatic profile was evaluated in liquid cultures using various carbon sources, a procedure that revealed lack of correlation on xylanase levels between the two cultivation modes and the inability of solid CMC cultures to fully unravel the cellulose degrading potential of the isolates. Strain SP24, showing more than 99 % 16S DNA similarity with Geobacillus sp. was further studied for its unique ability to simultaneously exhibit cellulase, xylanase, β-glucosidase and β-xylosidase activities. The first two enzymes were produced mainly extracellularly, while the β-glycosidic activities were primarily detected in the cytosol. Maximum enzyme production by this strain was attained using a combination of wheat bran and xylan in the growth medium. Bioreactor cultures showed that aeration was necessary for both enhanced growth and enzyme production. Aeration had a strong positive effect on cellulase production while it negatively affected expression of β-glucosidase. Xylanase and β-xylosidase production was practically unaffected by aeration levels.
Subject headings
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Keyword
- Biochemical screening
- Xylanase
- Geobacillus
- Aeration
- Cellulase
- Volcanic
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
- World Journal of Microbiology and Biotechnology (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Stathopoulou, P. ...
- Galanopoulou, A. ...
- Anasontzis, Geor ...
- Karagouni, A.D.
- Hatzinikolaou, D ...
- About the subject
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Biological Scien ...
- and Microbiology
- Articles in the publication
- World Journal of ...
- By the university
- Chalmers University of Technology
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
