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Modular synthetic b...
Modular synthetic biology toolkit for filamentous fungi
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- Mózsik, László (författare)
- Rijksuniversiteit Groningen,University of Groningen
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- Pohl, Carsten (författare)
- Technische Universität Berlin
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- Meyer, Vera (författare)
- Technische Universität Berlin
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- Bovenberg, R. A. L. (författare)
- DSM Biotechnology Centre,Rijksuniversiteit Groningen,University of Groningen
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- Nygård, Yvonne, 1986 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Driessen, Arnold J.M. (författare)
- Rijksuniversiteit Groningen,University of Groningen
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(creator_code:org_t)
- 2021-11-02
- 2021
- Engelska.
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Ingår i: ACS Synthetic Biology. - : American Chemical Society (ACS). - 2161-5063. ; 10:11, s. 2850-2861
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Abstract
Ämnesord
Stäng
- Filamentous fungi are highly productive cell factories, often used in industry for the production of enzymes and small bioactive compounds. Recent years have seen an increasing number of synthetic-biology-based applications in fungi, emphasizing the need for a synthetic biology toolkit for these organisms. Here we present a collection of 96 genetic parts, characterized in Penicillium or Aspergillus species, that are compatible and interchangeable with the Modular Cloning system. The toolkit contains natural and synthetic promoters (constitutive and inducible), terminators, fluorescent reporters, and selection markers. Furthermore, there are regulatory and DNA-binding domains of transcriptional regulators and components for implementing different CRISPR-based technologies. Genetic parts can be assembled into complex multipartite assemblies and delivered through genomic integration or expressed from an AMA1-sequence-based, fungal-replicating shuttle vector. With this toolkit, synthetic transcription units with established promoters, fusion proteins, or synthetic transcriptional regulation devices can be more rapidly assembled in a standardized and modular manner for novel fungal cell factories.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- Hybrid transcription factor
- Filamentous fungi
- Inducible promoter
- Synthetic biology toolkit
- Modular cloning
- Transcriptional regulation
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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