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Characterization of...
Characterization of CYP82 genes involved in the biosynthesis of structurally diverse benzylisoquinoline alkaloids in Corydalis yanhusuo
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- Liu, Xiuyu (författare)
- Henan University of Chinese Medicine
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- Ma, Ying (författare)
- China Academy of Chinese Medical Sciences
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- Bu, Junling (författare)
- China Academy of Chinese Medical Sciences
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- Lian, Conglong (författare)
- Henan University of Chinese Medicine
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- Ma, Rui (författare)
- Henan University of Chinese Medicine
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- Li, Qishuang (författare)
- China Academy of Chinese Medical Sciences
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- Jiao, Xiang, 1990 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Hu, Zhimin (författare)
- China Academy of Chinese Medical Sciences
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- Chen, Yun, 1978 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Chen, Suiqing (författare)
- Henan University of Chinese Medicine
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- Guo, Juan (författare)
- China Academy of Chinese Medical Sciences
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- Huang, Luqi (författare)
- China Academy of Chinese Medical Sciences
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(creator_code:org_t)
- 2024
- 2024
- Engelska.
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Ingår i: Plant Molecular Biology. - 0167-4412 .- 1573-5028. ; 114:2
- Relaterad länk:
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- Benzylisoquinoline alkaloids (BIAs) represent a significant class of secondary metabolites with crucial roles in plant physiology and substantial potential for clinical applications. CYP82 genes are involved in the formation and modification of various BIA skeletons, contributing to the structural diversity of compounds. In this study, Corydalis yanhusuo, a traditional Chinese medicine rich in BIAs, was investigated to identify the catalytic function of CYP82s during BIA formation. Specifically, 20 CyCYP82-encoding genes were cloned, and their functions were identified in vitro. Ten of these CyCYP82s were observed to catalyze hydroxylation, leading to the formation of protopine and benzophenanthridine scaffolds. Furthermore, the correlation between BIA accumulation and the expression of CyCYP82s in different tissues of C. yanhusuo was assessed their. The identification and characterization of CyCYP82s provide novel genetic elements that can advance the synthetic biology of BIA compounds such as protopine and benzophenanthridine, and offer insights into the biosynthesis of BIAs with diverse structures in C. yanhusuo.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
Nyckelord
- CyCYP82s
- Benzylisoquinoline alkaloids (BIAs)
- Functional characterization
- Corydalis yanhusuo
- Biosynthetic pathway
- Genetic elements
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Till lärosätets databas
- Av författaren/redakt...
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Liu, Xiuyu
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Ma, Ying
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Bu, Junling
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Lian, Conglong
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Ma, Rui
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Li, Qishuang
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visa fler...
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Jiao, Xiang, 199 ...
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Hu, Zhimin
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Chen, Yun, 1978
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Chen, Suiqing
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Guo, Juan
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Huang, Luqi
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Organisk kemi
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Plant Molecular ...
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Chalmers tekniska högskola