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Freeze-Drying as Sa...
Freeze-Drying as Sample Preparation for Micellar Electrokinetic Capillary Chromatography-Electrochemical Separations of Neurochemicals in Drosophila Brains
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- Berglund, E. C. (author)
- Göteborgs universitet,University of Gothenburg
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- Kuklinski, N. J. (author)
- Pennsylvania State University
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- Karagunduz, E. (author)
- Göteborgs universitet,University of Gothenburg
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- Ucar, Kubra, 1986 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Hanrieder, Jörg, 1980 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Ewing, Andrew, 1957 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- 2013-02-22
- 2013
- English.
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In: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 85:5, s. 2841-2846
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http://dx.doi.org/10...
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Abstract
Subject headings
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- Micellar electrokinetic capillary chromatography with electrochemical detection has been used to quantify biogenic amines in freeze-dried brains of Drosophila melanogaster. Freeze-drying samples offers a way to preserve the biological sample while making dissection of these tiny samples easier and faster. Fly samples were extracted in cold acetone and dried in a rotary evaporator. Extraction and drying times were optimized in order to avoid contamination by red pigment from the fly eyes and still have intact brain structures. Single freeze-dried fly brain samples were found to produce representative electropherograms as a single hand-dissected brain sample. With utilization of the faster dissection time that freeze-drying affords, the number of brains in a fixed homogenate volume can be increased to concentrate the sample. Thus, concentrated brain samples containing five or fifteen preserved brains were analyzed for their neurotransmitter content, and four analytes; N-acetyloctopamine, N-acetylserotonin, N-acetyltyramine, and N-acetyldopamine were found to correspond well with previously reported values.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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