Structure-function analysis of CYP719As involved in methylenedioxy bridge-formation in the biosynthesis of benzylisoquinoline alkaloids and its de novo production

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Structure-function analysis of CYP719As involved in methylenedioxy bridge-formation in the biosynthesis of benzylisoquinoline alkaloids and its de novo production

Liu, Xiuyu (author): Henan University of Chinese Medicine,China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences

Jiao, Xiang, 1990 (author): Chalmers tekniska högskola,Chalmers University of Technology

Cheng, Yatian (author): China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences

Ma, Ying (author): China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences

Bu, Junling (author): China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences

Jin, Baolong (author): China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences

Li, Qishuang (author): China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences

Hu, Zhimin (author): China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences

Tang, Jinfu (author): China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences

Lai, Changjiangsheng (author): China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences

Wang, Jian (author): China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences

Cui, Guanghong (author): China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences

Chen, Yun, 1978 (author): Chalmers tekniska högskola,Chalmers University of Technology

Guo, Juan (author): China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences

Huang, Luqi (author): China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences

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2023-02-03
2023
English.
In: Microbial Cell Factories. - : Springer Science and Business Media LLC. - 1475-2859. ; 22:1

Related links:: https://research.cha... (primary) (free); show more...; https://research.cha...; https://doi.org/10.1...; show less...

Journal article (peer-reviewed)

Abstract Subject headings

Benzylisoquinoline alkaloids (BIAs) are a type of secondary metabolite with clinical application value. (S)-stylopine is a special BIA which contains methylenedioxy bridge structures. CYP719As could catalyze the methylenedioxy bridge-formation on the A or D rings of protoberberine alkaloids, while displaying significant substrate regiospecificity. To explore the substrate preference of CYP719As, we cloned and identified five CyCYP719A candidates from Corydalis yanhusuo. Two CyCYP719As (CyCYP719A39 and CyCYP719A42) with high catalytic efficiency for the methylenedioxy bridge-formation on the D or A rings were characterized, respectively. The residues (Leu 294 for CyCYP719A42 and Asp 289 for CyCYP719A39) were identified as the key to controlling the regioselectivity of CYP719As affecting the methylenedioxy bridge-formation on the A or D rings by homology modeling and mutation analysis. Furthermore, for de novo production of BIAs, CyCYP719A39, CyCYP719A42, and their mutants were introduced into the (S)-scoulerine-producing yeast to produce 32 mg/L (S)-stylopine. These results lay a foundation for understanding the structure-function relationship of CYP719A-mediated methylenedioxy bridge-formation and provide yeast strains for the BIAs production by synthetic biology.

Subject headings

NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)
NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)

Keyword

Methylenedioxy bridge-formation
Regiospecificity
Corydalis yanhusuo
Synthetic biology
Benzylisoquinoline alkaloids (BIAs)
CyCYP719As

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art (subject category)
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By the author/editor: Liu, Xiuyu; Jiao, Xiang, 199 ...; Cheng, Yatian; Ma, Ying; Bu, Junling; Jin, Baolong; show more...; Li, Qishuang; Hu, Zhimin; Tang, Jinfu; Lai, Changjiangs ...; Wang, Jian; Cui, Guanghong; Chen, Yun, 1978; Guo, Juan; Huang, Luqi; show less...

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NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

ENGINEERING AND TECHNOLOGY: ENGINEERING AND ...; and Industrial Biote ...; and Biocatalysis and ...

NATURAL SCIENCES: NATURAL SCIENCES; and Chemical Science ...; and Organic Chemistr ...

Articles in the publication: Microbial Cell F ...

By the university: Chalmers University of Technology

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