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STRUCTURAL CHARACTE...
STRUCTURAL CHARACTERIZATION OF PNA-DNA DUPLEXES BY NMR - EVIDENCE FOR DNA IN A B-LIKE CONFORMATION
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Leijon, M. (author)
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Graslund, A. (author)
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Nielsen, P. E. (author)
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Buchardt, O. (author)
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- Nordén, Bengt, 1945 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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Kristensen, S. M. (author)
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Eriksson, M. (author)
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(creator_code:org_t)
- 2002-05-01
- 1994
- English.
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In: Biochemistry. - : American Chemical Society (ACS). - 1520-4995 .- 0006-2960. ; 33:33, s. 9820-9825
- Related links:
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https://doi.org/10.1...
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Abstract
Subject headings
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- The nucleic acid analogues PNA (peptide nucleic acids) hybridize with DNA of complementary sequence. The solution structures of two PNA-DNA duplexes, H-(GCTATGTC)-NH2.d(GACATAGC) and H-(GTAGATCACT)-NH2.d(AGTGATCTAC), have been studied by H-1 NMR. It was found that the PNA-DNA hybrids are base paired by hydrogen bonds, most likely of the Watson-Crick type. From two-dimensional NOESY and COSY results it is concluded that the DNA strand in the PNA-DNA complex adopts a B-like structure with the deoxyribose sugars in the C2'-endo conformation.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- spectra
- thymine-substituted polyamide
- assignment
- spectroscopy
- proton resonances
- nuclear magnetic-resonance
- strand-displacement
- suppression
- oligonucleotides
- coupling-constants
Publication and Content Type
- art (subject category)
- ref (subject category)
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