Metabolic engineering and transcriptomic analysis of Saccharomyces cerevisiae producing p-coumaric acid from xylose

Borja, Gheorghe M. (author): Danmarks Tekniske Universitet,Technical University of Denmark

Rodriguez, A. (author): Danmarks Tekniske Universitet,Technical University of Denmark,Köpenhamns universitet,University of Copenhagen

Campbell, Kate, 1987 (author): Chalmers tekniska högskola,Chalmers University of Technology

Borodina, I. (author): Danmarks Tekniske Universitet,Technical University of Denmark

Chen, Yun, 1978 (author): Chalmers tekniska högskola,Chalmers University of Technology

Nielsen, Jens B, 1962 (author): BioInnovation Institute (BII),Köpenhamns universitet,University of Copenhagen,Chalmers tekniska högskola,Chalmers University of Technology,Danmarks Tekniske Universitet,Technical University of Denmark

(creator_code:org_t)

2019-11-05
2019
English.
In: Microbial Cell Factories. - : Springer Science and Business Media LLC. - 1475-2859. ; 18:1

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Abstract Subject headings

Background: Aromatic amino acids and their derivatives are valuable chemicals and are precursors for different industrially compounds. p-Coumaric acid is the main building block for complex secondary metabolites in commercial demand, such as flavonoids and polyphenols. Industrial scale production of this compound from yeast however remains challenging. Results: Using metabolic engineering and a systems biology approach, we developed a Saccharomyces cerevisiae platform strain able to produce 242 mg/L of p-coumaric acid from xylose. The same strain produced only 5.35 mg/L when cultivated with glucose as carbon source. To characterise this platform strain further, transcriptomic analysis was performed, comparing this strain's growth on xylose and glucose, revealing a strong up-regulation of the glyoxylate pathway alongside increased cell wall biosynthesis and unexpectedly a decrease in aromatic amino acid gene expression when xylose was used as carbon source. Conclusions: The resulting S. cerevisiae strain represents a promising platform host for future production of p-coumaric using xylose as a carbon source.

TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Bioprocessteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bioprocess Technology (hsv//eng)
NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Annan industriell bioteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Other Industrial Biotechnology (hsv//eng)

By the author/editor: Borja, Gheorghe ...; Rodriguez, A.; Campbell, Kate, ...; Borodina, I.; Chen, Yun, 1978; Nielsen, Jens B, ...

About the subject

ENGINEERING AND TECHNOLOGY: ENGINEERING AND ...; and Industrial Biote ...; and Bioprocess Techn ...

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Microbiology

ENGINEERING AND TECHNOLOGY: ENGINEERING AND ...; and Industrial Biote ...; and Other Industrial ...

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