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Lipid-Based Passiva...
Lipid-Based Passivation in Nanofluidics
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- Persson, Fredrik, 1979 (författare)
- Uppsala universitet,Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),Beräknings- och systembiologi,Science for Life Laboratory, SciLifeLab
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- Fritzsche, Joachim, 1977 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)
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- Mir, K. U. (författare)
- Wellcome Trust Centre for Human Genetics
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- Modesti, M. (författare)
- Centre de Recherche en Cancerologie de Marseille
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- Westerlund, Fredrik, 1978 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Tegenfeldt, Jonas (författare)
- Gothenburg University,Göteborgs universitet,Lund University,Lunds universitet,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH,Institutionen för fysik (GU),Department of Physics (GU)
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(creator_code:org_t)
- 2012-04-06
- 2012
- Engelska.
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Ingår i: Nano Letters. - : American Chemical Society (ACS). - 1530-6992 .- 1530-6984. ; 12:5, s. 2260-2265
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Abstract
Ämnesord
Stäng
- Stretching DNA in nanochannels is a useful tool for direct, visual studies of genomic DNA at the single molecule level. To facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We demonstrate virtually complete long-term passivation of nanochannel surfaces to a range of relevant reagents, including streptavidin-coated quantum dots, RecA proteins, and RecA-DNA complexes. We show that the performance of the lipid bilayer is significantly better than that of standard bovine serum albumin-based passivation. Finally, we show how the passivated devices allow us to monitor single DNA cleavage events during enzymatic degradation by DNase I. We expect that our approach will open up for detailed, systematic studies of a wide range of protein-DNA interactions with high spatial and temporal resolution.
Ämnesord
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Nanoteknik -- Nanoteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Nano-technology -- Nano-technology (hsv//eng)
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
Nyckelord
- lipid bilayer
- Nanofluidics
- antifouling
- single molecules
- passivation
- protein−DNA interactions
- Nanofluidics
- passivation
- antifouling
- lipid bilayer
- protein-DNA
- interactions
- single molecules
- Nanofluidics; passivation; antifouling; lipid bilayer; protein−DNA interactions; single molecules
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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