id:"swepub:oai:slubar.slu.se:101175"
Search: id:"swepub:oai:slubar.slu.se:101175" > Efficient RNA silen...
- 1 of 1
- Previous record
- Next record
- To hitlist
Efficient RNA silencing suppression activity of Potato Mop-Top Virus 8K protein is driven by variability and positive selection
-
- Kalyandurg, Pruthvi Balachandra (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology
-
- Tahmasebi, Aminallah (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology,Shiraz University
-
- Ramesh, Vetukuri (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Växtskyddsbiologi,Department of Plant Protection Biology
- show more...
- show less...
- (creator_code:org_t)
- Elsevier BV, 2019
- 2019
- English.
- In: Virology. - : Elsevier BV. - 0042-6822 .- 1178-122X. ; 535, s. 111-121
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Previously, we investigated the evolution of Potato mop-top virus (PMTV) ORFs. Results indicate that positive selection acts exclusively on an ORF encoding the 8K protein, a weak viral suppressor of RNA silencing (VSR). However, how the extraordinary variability contributes to 8K-mediated RNA silencing suppression remains unknown. Here, we characterized the RNA silencing suppression activity of the 8K protein from seven diverse isolates. We show that 8K encoded by isolate P1 exhibits stronger RNA silencing suppression activity than the 8K protein from six other isolates. Mutational analyses revealed that Ser-50 is critical for these differences. By comparing small RNA profiles we found a lower abundance of siRNAs with U residue at the 5'-terminus after expression of the P1 8K compared to expression of 8K from isolate P125, an isolate with weak VSR activity. These results provide new clues as to the role of positive selection in shaping activities of VSRs.
Subject headings
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Jordbruksvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Agricultural Science (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Kalyandurg, Prut ...
- Tahmasebi, Amina ...
- Ramesh, Vetukuri
- Kushwaha, Sandee ...
- Grenville-Briggs ...
- Savenkov, Eugene
- About the subject
-
- AGRICULTURAL SCIENCES
- AGRICULTURAL SCI ...
- and Agriculture Fore ...
- and Agricultural Sci ...
- Articles in the publication
- Virology
- By the university
- Swedish University of Agricultural Sciences
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
