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Sökning: id:"swepub:oai:slubar.slu.se:122283" > Core genome multilo...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003754naa a2200349 4500
001oai:slubar.slu.se:122283
003SwePub
008240410s2023 | |||||||||||000 ||eng|
024a https://res.slu.se/id/publ/1222832 URI
024a https://doi.org/10.1016/j.vetmic.2023.1097552 DOI
040 a (SwePub)slu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
100a Jonare, Livu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences4 aut
2451 0a Core genome multilocus sequence typing (cgMLST) confirms systemic spread of avian pathogenic Escherichia coli (APEC) in broilers with cellulitis
264 1c 2023
264 1b Elsevier,c 2024
520 a Broiler cellulitis has emerged as an important cause of economic losses for farmers and slaughter plants from carcass condemnation at processing. Avian pathogenic Escherichia coli (APEC) has been identified as the main causative agent. The aim was to characterize E. coli isolated from cellulitis and organs in broilers at slaughter by whole genome sequencing analysis to study if systemic spread could be confirmed. Isolates were collected postmortem from 101 carcasses condemned due to dermatitis/cellulitis from five commercial farms and six flocks. Forty-six isolates were characterised to determine serotypes, sequence types and virulence-associated genes. Analysis by cgMLST was performed to study the genetic similarity between isolates from the same broiler, among birds from the same flock and between flocks. Escherichia coli was isolated from 90% of birds from subcutaneous samples. In 20 broilers, E. coli was isolated from organs in pure culture or mixed with sparse growth of other bacteria. In eight of these, there were post-mortem findings suggestive of systemic bacterial spread. The majority of the isolates from the same bird and flock belonged to the same serotype and sequence type and were genetically indistinguishable, but differed when compared between flocks. Common APEC virulence genes, i.e. chuA, fyuA, hlyF, iroN, irp2, iss, ompT, sitA, TerC, TraT, were present in > 87% of the isolates. We conclude that evidence of systemic spread of E. coli from cellulitis was present in some birds at time of slaughter but cannot be reliably detected at meat inspection.
650 7a LANTBRUKSVETENSKAPERx Veterinärmedicinx Patobiologi0 (SwePub)403022 hsv//swe
650 7a AGRICULTURAL SCIENCESx Veterinary Sciencex Pathobiology0 (SwePub)403022 hsv//eng
650 7a LANTBRUKSVETENSKAPERx Veterinärmedicinx Klinisk vetenskap0 (SwePub)403032 hsv//swe
650 7a AGRICULTURAL SCIENCESx Veterinary Sciencex Clinical Science0 (SwePub)403032 hsv//eng
700a Hansson, Ingridu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för biomedicin och veterinär folkhälsovetenskap (BVF),Department of Biomedical Science and Veterinary Public Health4 aut0 (Swepub:slu)52558
700a Jansson, Desiréeu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences,National Veterinary Institute (SVA)4 aut0 (Swepub:slu)51571
710a Sveriges lantbruksuniversitetb Institutionen för kliniska vetenskaper (KV)4 org
710a Sveriges lantbruksuniversitet
773t Veterinary Microbiologyg 282q 282x 0378-1135x 1873-2542
856u https://pub.epsilon.slu.se/id/eprint/31086/contentsx primaryx Raw objectx freey FULLTEXT
8564 8u https://res.slu.se/id/publ/122283
8564 8u https://doi.org/10.1016/j.vetmic.2023.109755

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Jonare, Liv
Hansson, Ingrid
Jansson, Desirée
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